The magic cocktail: ampicillin and biosynthesized gold nanoparticles synergism against staphylococcus aureus

Marco Oliveira; Silvia Soares; Sara Sá; Álvaro Gestoso; Miguel Correa-Duarte; Pilar Baylina; Rúben Fernandes; Carla F. Pereira

doi:10.3390/app131910934

The magic cocktail: ampicillin and biosynthesized gold nanoparticles synergism against staphylococcus aureus

Marco Oliveira
, Silvia Soares
, Sara Sá
, Álvaro Gestoso
, Miguel Correa-Duarte
, Pilar Baylina
, Rúben Fernandes^*
, Carla F. Pereira^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

40 Downloads

Abstract

Gold nanoparticles (AuNPs) have garnered attention as a potential alternative to conventional antibiotics due to their innovative antibacterial properties. This study demonstrates the successful production of biosynthetic gold nanoparticles (bAuNPs) using Pseudomonas aeruginosa (P. aeruginosa) as spherical nanostructures at 58 °C for 24 h, under alkaline pH (9.0). The successful synthesis of bAuNPs was confirmed through UV-Vis spectroscopy, exhibiting a characteristic peak within the 500–600 nm wavelength range, and the evaluation of the main functional groups and morphology were stressed by Fourier Transform Infrared Spectroscopy (FT-IR) and Transmission Electron Microscopy (TEM), respectively. Subsequently, the synthesized bAuNPs were combined with low concentrations of ampicillin and evaluated against Methicillin-Susceptible Staphylococcus aureus (MSSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) through the classical serial dilution method. This innovative approach holds the potential to address the escalating issue of antibiotic resistance, providing a viable and sustainable solution.

Original language	English
Article number	10934
Number of pages	13
Journal	Applied Sciences
Volume	13
Issue number	19
DOIs	https://doi.org/10.3390/app131910934
Publication status	Published - Oct 2023

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Ampicillin
Antibiotic resistance
Biosynthetic gold nanoparticles
Synergism

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76607717Final published version, 1.91 MBLicence: CC BY

http://hdl.handle.net/10400.14/42918Licence: CC BY

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title = "The magic cocktail: ampicillin and biosynthesized gold nanoparticles synergism against staphylococcus aureus",

abstract = "Gold nanoparticles (AuNPs) have garnered attention as a potential alternative to conventional antibiotics due to their innovative antibacterial properties. This study demonstrates the successful production of biosynthetic gold nanoparticles (bAuNPs) using Pseudomonas aeruginosa (P. aeruginosa) as spherical nanostructures at 58 °C for 24 h, under alkaline pH (9.0). The successful synthesis of bAuNPs was confirmed through UV-Vis spectroscopy, exhibiting a characteristic peak within the 500–600 nm wavelength range, and the evaluation of the main functional groups and morphology were stressed by Fourier Transform Infrared Spectroscopy (FT-IR) and Transmission Electron Microscopy (TEM), respectively. Subsequently, the synthesized bAuNPs were combined with low concentrations of ampicillin and evaluated against Methicillin-Susceptible Staphylococcus aureus (MSSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) through the classical serial dilution method. This innovative approach holds the potential to address the escalating issue of antibiotic resistance, providing a viable and sustainable solution.",

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note = "Publisher Copyright: {\textcopyright} 2023 by the authors.",

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doi = "10.3390/app131910934",

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T2 - ampicillin and biosynthesized gold nanoparticles synergism against staphylococcus aureus

AU - Oliveira, Marco

AU - Soares, Silvia

AU - Sá, Sara

AU - Gestoso, Álvaro

AU - Correa-Duarte, Miguel

AU - Baylina, Pilar

AU - Fernandes, Rúben

AU - Pereira, Carla F.

PY - 2023/10

Y1 - 2023/10

N2 - Gold nanoparticles (AuNPs) have garnered attention as a potential alternative to conventional antibiotics due to their innovative antibacterial properties. This study demonstrates the successful production of biosynthetic gold nanoparticles (bAuNPs) using Pseudomonas aeruginosa (P. aeruginosa) as spherical nanostructures at 58 °C for 24 h, under alkaline pH (9.0). The successful synthesis of bAuNPs was confirmed through UV-Vis spectroscopy, exhibiting a characteristic peak within the 500–600 nm wavelength range, and the evaluation of the main functional groups and morphology were stressed by Fourier Transform Infrared Spectroscopy (FT-IR) and Transmission Electron Microscopy (TEM), respectively. Subsequently, the synthesized bAuNPs were combined with low concentrations of ampicillin and evaluated against Methicillin-Susceptible Staphylococcus aureus (MSSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) through the classical serial dilution method. This innovative approach holds the potential to address the escalating issue of antibiotic resistance, providing a viable and sustainable solution.

AB - Gold nanoparticles (AuNPs) have garnered attention as a potential alternative to conventional antibiotics due to their innovative antibacterial properties. This study demonstrates the successful production of biosynthetic gold nanoparticles (bAuNPs) using Pseudomonas aeruginosa (P. aeruginosa) as spherical nanostructures at 58 °C for 24 h, under alkaline pH (9.0). The successful synthesis of bAuNPs was confirmed through UV-Vis spectroscopy, exhibiting a characteristic peak within the 500–600 nm wavelength range, and the evaluation of the main functional groups and morphology were stressed by Fourier Transform Infrared Spectroscopy (FT-IR) and Transmission Electron Microscopy (TEM), respectively. Subsequently, the synthesized bAuNPs were combined with low concentrations of ampicillin and evaluated against Methicillin-Susceptible Staphylococcus aureus (MSSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) through the classical serial dilution method. This innovative approach holds the potential to address the escalating issue of antibiotic resistance, providing a viable and sustainable solution.

KW - Ampicillin

KW - Antibiotic resistance

KW - Biosynthetic gold nanoparticles

KW - Synergism

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DO - 10.3390/app131910934

M3 - Article

AN - SCOPUS:85174172604

SN - 2076-3417

VL - 13

JO - Applied Sciences

JF - Applied Sciences

IS - 19

M1 - 10934

ER -

The magic cocktail: ampicillin and biosynthesized gold nanoparticles synergism against staphylococcus aureus

Abstract

UN SDGs

Keywords

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