The NAD interactome: identification of putative new NAD-binding proteins

Sara Duarte-Pereira, Sérgio Matos, José Luís Oliveira, Raquel Silva

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Nicotinamide adenine dinucleotide (NAD) is an essential metabolite in normal cellular physiology and its deregulation may lead to several pathological conditions. NAD interacts with a vast number of proteins, acting as a coenzyme, as a substrate and regulating the interaction between proteins. The goals of this study were to characterize the proteins involved in NAD metabolism and to identify putative new NAD regulated proteins. Using an in silico approach, we first defined a NAD-binding dataset, that we characterized through pathway enrichment analysis and protein structural domains analysis. We then screened the full human proteome and further analyzed a selection of potential NAD-binding proteins. This global study of the NAD interactome resulted in the identification of new potentially NAD-binding proteins (NADPBs), including TRPC3 and a few isoforms of DGA kinases, which are involved in calcium signaling. NADBPs participate in several metabolic pathways and signaling processes in the cell, while proteins interacting with NADPBs are mostly involved in signaling pathways, including pathways related to disease, namely three major neurodegenerative diseases, Alzheimer’s, Huntington’s, and Parkinson’s.
Original languageEnglish
Title of host publicationPractical Applications of Computational Biology and Bioinformatics, 16th International Conference, PACBB 2022
EditorsFlorentino Fdez-Riverola, Miguel Rocha, Mohd Saberi Mohamad, Simona Caraiman, Ana Belén Gil-González
Place of PublicationCham
PublisherSpringer Nature Switzerland AG
Pages43-54
Number of pages12
Volume553
ISBN (Electronic)9783031170249
ISBN (Print)9783031170232
DOIs
Publication statusPublished - 2023
Event16th International Conference on Practical Applications of Computational Biology & Bioinformatics - L'Aquila, Italy
Duration: 13 Jul 202215 Jul 2022

Publication series

NameLecture Notes in Networks and Systems
Volume553
ISSN (Print)2367-3370
ISSN (Electronic)2367-3389

Conference

Conference16th International Conference on Practical Applications of Computational Biology & Bioinformatics
Abbreviated titlePACBB 2022
Country/TerritoryItaly
CityL'Aquila
Period13/07/2215/07/22

Keywords

  • Nicotinamide Adenine Dinucleotide (NAD)
  • Protein-protein interactions (PPIs)
  • Protein domains

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