TY - JOUR
T1 - The use of an oil-absorber-bioscrubber system during biodegradation of sequentially alternating loadings of 1,2-dichloroethane and fluorobenzene in a waste gas
AU - Koutinas, Michalis
AU - Baptista, Inês I. R.
AU - Meniconi, Andrea
AU - Peeva, Ludmila G.
AU - Mantalaris, Athanasios
AU - Castro, Paula M. L.
AU - Livingston, Andrew G.
PY - 2007/11
Y1 - 2007/11
N2 - This work seeks to improve the robustness of vapour phase bioscrubbing by applying an absorber prior to a bioscrubber during the dynamic treatment of sequentially alternating loads of inhibitory pollutants. Rhizobiales sp. strain F11 and Xanthobacter autotrophicus sp. GJ10, exhibiting specific degradation capabilities for fluorobenzene (FB) and 1,2-dichloroethane (DCE), respectively, were used as a compound-strain model system. The stability of a combined oil-absorber-bioscrubber (OAB) was compared to the stability of a bioscrubber only (BO) system, during sequentially alternating periods (duration 3-6 d) of FB and DCE in the gas feed. The OAB achieved > 66 % degradation of FB, while in the BO system the FB removal efficiency dropped to 0% upon restoring FB feed after a 3 d FB starvation period. Following 6 d of FB starvation the BO failed to recover within 10 d, while the OAB required only 2 d to recover. In contrast, during the DCE treatment periods the OAB system did not show any advantage over the BO system. Further investigation showed that the F- (a main metabolic product from FB degradation) has a strong inhibitory effect on strain GJ10 even at concentrations below 50 mg L- 1. In the OAB system the inhibitory effect persisted for longer periods due to the absorber, which continued to supply FB to the system, and caused deterioration in the DCE removal efficiency. The inhibition of the microbial culture was confirmed by fluorescence in situ hybridisation (FISH), which showed that the activity of cells was reduced when only FB was fed. The results of this study have shown that in the presence of an inhibitory metabolic product the OAB system may not effectively improve the biological treatment of waste gas during sequential alternations in the feed of inhibitory pollutants.
AB - This work seeks to improve the robustness of vapour phase bioscrubbing by applying an absorber prior to a bioscrubber during the dynamic treatment of sequentially alternating loads of inhibitory pollutants. Rhizobiales sp. strain F11 and Xanthobacter autotrophicus sp. GJ10, exhibiting specific degradation capabilities for fluorobenzene (FB) and 1,2-dichloroethane (DCE), respectively, were used as a compound-strain model system. The stability of a combined oil-absorber-bioscrubber (OAB) was compared to the stability of a bioscrubber only (BO) system, during sequentially alternating periods (duration 3-6 d) of FB and DCE in the gas feed. The OAB achieved > 66 % degradation of FB, while in the BO system the FB removal efficiency dropped to 0% upon restoring FB feed after a 3 d FB starvation period. Following 6 d of FB starvation the BO failed to recover within 10 d, while the OAB required only 2 d to recover. In contrast, during the DCE treatment periods the OAB system did not show any advantage over the BO system. Further investigation showed that the F- (a main metabolic product from FB degradation) has a strong inhibitory effect on strain GJ10 even at concentrations below 50 mg L- 1. In the OAB system the inhibitory effect persisted for longer periods due to the absorber, which continued to supply FB to the system, and caused deterioration in the DCE removal efficiency. The inhibition of the microbial culture was confirmed by fluorescence in situ hybridisation (FISH), which showed that the activity of cells was reduced when only FB was fed. The results of this study have shown that in the presence of an inhibitory metabolic product the OAB system may not effectively improve the biological treatment of waste gas during sequential alternations in the feed of inhibitory pollutants.
KW - 1,2-Dichloroethane
KW - Absorption
KW - Bioreactors
KW - Environment
KW - Fluorobenzene
KW - Molecular biology
UR - http://www.scopus.com/inward/record.url?scp=34748911718&partnerID=8YFLogxK
U2 - 10.1016/j.ces.2007.06.040
DO - 10.1016/j.ces.2007.06.040
M3 - Article
AN - SCOPUS:34748911718
SN - 0009-2509
VL - 62
SP - 5989
EP - 6001
JO - Chemical Engineering Science
JF - Chemical Engineering Science
IS - 21
ER -