Timing carbon turnover (δ13C) in weaned piglet’s brain by IRMS

Mayra A. D. Saleh*, Luan S. dos Santos, Dirlei A. Berto, Alessandro B. Amorim, Vladimir E. Costa, Carlos Ducatti

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Isotope-ratio mass spectrometry (IRMS) is a potential tool that provides time-integrated estimate of assimilated and not just ingested nutrients. This feature turned possible its application to evaluate the effects of dietary nucleotides and glutamate on carbon turnover (δ13C) in the brain of weanling piglets. Eighty-seven piglets weaned at 21-day-old were used, being three piglets slaughtered at day-0 (prior to diet-switch) and, the remaining 84 randomly assigned in a complete block design with a 2 x 2 factorial arrangement of treatments (two nucleotide levels: 0% and 0.1% and, two glutamate levels: 0% and 1%). On trial days 3, 6, 9, 14, 21, 35 and 49, three piglets per treatment were also slaughtered. The samples were analyzed by IRMS and adjusted to the first-order equation by a nonlinear regression analysis using NLIN procedure of SAS, in order to obtain the exponential graphics. The carbon turnover (t95%) verified for cerebral tissue was faster (P<0.05) for diet containing glutamate in comparison to other diets, supporting the fact that glutamate contributed to develop the piglets’ brain, due to the fastest incorporation of13C-atoms in this tissue at post-weaning phase, despite the energy deficit experienced by them.
Original languageEnglish
Pages (from-to)2469-2478
Number of pages10
JournalAnais da Academia Brasileira de Ciencias
Volume90
Issue number2
DOIs
Publication statusPublished - 2018
Externally publishedYes

Keywords

  • Additives
  • Amino acids
  • Isotopic dilution
  • Stable isotopes
  • Swine

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