Tricarboxylic enzymes in ripening strawberry fruits

P. P.M. Iannetta, N. Medina Escobar, H. A. Ross, E. S. Souleyre, R. Hancock, J. Fairlie, H. V. Davies

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Two-dimensional (2D; native/SDS) polyacrylamide gel electrophoresis (PAGE) was used to identify the N-termini of two salt extractable proteins whose translation is up-regulated in mature strawberry (Elsanta) fruits. The N-termini had a high sequence similarity to enzymes of the tricarboxylic acid cycle, mitochondrial citrate synthase and malate dehydrogenase. In correlation with the 2D-PAGE data, levels of citrate and malate and associated enzyme activities increased as fruit ripened. However, northern analysis has revealed that the RNA levels for both enzymes were highest in flowers and immature green fruits. A post-translational block may be operating in flowers and immature fruits.

Original languageEnglish
Title of host publicationIV International Strawberry Symposium
PublisherInternational Society for Horticultural Science
Pages731-733
Number of pages3
ISBN (Print)9789066057753
DOIs
Publication statusPublished - 2002

Publication series

NameActa Horticulturae
Volume567
ISSN (Print)0567-7572

Keywords

  • Fruit ripening
  • Organic acids
  • Protein sequencing
  • Strawberry
  • TCA

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