Ultrasound-assisted extraction of bioactive compounds from goji berries: optimization, bioactivity, and intestinal permeability assessment

Filipa Teixeira, Ana Margarida Silva, Stefania Sut, Stefano Dall'Acqua, Oscar L. Ramos, Alessandra B. Ribeiro, Ricardo Ferraz, Cristina Delerue-Matos, Francisca Rodrigues*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
22 Downloads

Abstract

Lycium barbarum L. berries have a remarkable chemical composition and extensive biological activities, being a valuable component of health and nutraceutical practices. Nevertheless, a deep insight on the intestinal permeation of the pro-healthy bioactive compounds is urgently needed to predict the real effects on human body. This study attempted, for the first time, to optimize the Ultrasound-Assisted Extraction (UAE) of goji berries using a Response Surface Methodology approach and establish the intestinal permeation of the principal pro-healthy compounds. The optimal extraction conditions were a solid:liquid ratio of 8.75 % for 56.21 min, using an intensity of 59.05 W/m2. The optimal extract displayed a remarkable antioxidant capacity, with LC/DAD-ESI-MS analysis unveiled a diverse phytochemical profile, encompassing different compounds (e.g. glu-lycibarbarspermidine F, 2-glu-kukoamine, rutin, 3,5-dicaffeoylquinic acid). The intestinal co-culture model demonstrated that glu-lycibarbarspermidine F (isomer 2) (73.70 %), 3,5-dicaffeoylquinic acid (52.66 %), and isorhamnetin-3-O-rutinoside (49.31 %) traversed the intestinal cell layer, exerting beneficial health-promoting effects.

Original languageEnglish
Article number114502
Number of pages13
JournalFood Research International
Volume188
DOIs
Publication statusPublished - Jul 2024

Keywords

  • Intestinal permeation
  • Lycium barbarum
  • Phenolic compounds
  • Response surface methodology
  • Ultrasound-assisted extraction

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