TY - JOUR
T1 - Ultrasound-assisted extraction of bioactive compounds from goji berries
T2 - optimization, bioactivity, and intestinal permeability assessment
AU - Teixeira, Filipa
AU - Silva, Ana Margarida
AU - Sut, Stefania
AU - Dall'Acqua, Stefano
AU - Ramos, Oscar L.
AU - Ribeiro, Alessandra B.
AU - Ferraz, Ricardo
AU - Delerue-Matos, Cristina
AU - Rodrigues, Francisca
N1 - Publisher Copyright:
© 2024 The Author(s)
PY - 2024/7
Y1 - 2024/7
N2 - Lycium barbarum L. berries have a remarkable chemical composition and extensive biological activities, being a valuable component of health and nutraceutical practices. Nevertheless, a deep insight on the intestinal permeation of the pro-healthy bioactive compounds is urgently needed to predict the real effects on human body. This study attempted, for the first time, to optimize the Ultrasound-Assisted Extraction (UAE) of goji berries using a Response Surface Methodology approach and establish the intestinal permeation of the principal pro-healthy compounds. The optimal extraction conditions were a solid:liquid ratio of 8.75 % for 56.21 min, using an intensity of 59.05 W/m2. The optimal extract displayed a remarkable antioxidant capacity, with LC/DAD-ESI-MS analysis unveiled a diverse phytochemical profile, encompassing different compounds (e.g. glu-lycibarbarspermidine F, 2-glu-kukoamine, rutin, 3,5-dicaffeoylquinic acid). The intestinal co-culture model demonstrated that glu-lycibarbarspermidine F (isomer 2) (73.70 %), 3,5-dicaffeoylquinic acid (52.66 %), and isorhamnetin-3-O-rutinoside (49.31 %) traversed the intestinal cell layer, exerting beneficial health-promoting effects.
AB - Lycium barbarum L. berries have a remarkable chemical composition and extensive biological activities, being a valuable component of health and nutraceutical practices. Nevertheless, a deep insight on the intestinal permeation of the pro-healthy bioactive compounds is urgently needed to predict the real effects on human body. This study attempted, for the first time, to optimize the Ultrasound-Assisted Extraction (UAE) of goji berries using a Response Surface Methodology approach and establish the intestinal permeation of the principal pro-healthy compounds. The optimal extraction conditions were a solid:liquid ratio of 8.75 % for 56.21 min, using an intensity of 59.05 W/m2. The optimal extract displayed a remarkable antioxidant capacity, with LC/DAD-ESI-MS analysis unveiled a diverse phytochemical profile, encompassing different compounds (e.g. glu-lycibarbarspermidine F, 2-glu-kukoamine, rutin, 3,5-dicaffeoylquinic acid). The intestinal co-culture model demonstrated that glu-lycibarbarspermidine F (isomer 2) (73.70 %), 3,5-dicaffeoylquinic acid (52.66 %), and isorhamnetin-3-O-rutinoside (49.31 %) traversed the intestinal cell layer, exerting beneficial health-promoting effects.
KW - Intestinal permeation
KW - Lycium barbarum
KW - Phenolic compounds
KW - Response surface methodology
KW - Ultrasound-assisted extraction
UR - http://www.scopus.com/inward/record.url?scp=85192831097&partnerID=8YFLogxK
U2 - 10.1016/j.foodres.2024.114502
DO - 10.1016/j.foodres.2024.114502
M3 - Article
C2 - 38823845
AN - SCOPUS:85192831097
SN - 0963-9969
VL - 188
JO - Food Research International
JF - Food Research International
M1 - 114502
ER -