Untargeted metabolomics relative quantification by swath mass spectrometry applied to cerebrospinal fluid

Vera M. Mendes, Margarida Coelho, Bruno Manadas*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Citations (Scopus)

Abstract

Cerebrospinal fluid (CSF) has been considered the key source for the search of biomarkers, in particular for neurological diseases, such as Alzheimer’s and Parkinson’s disease, since it reflects the state of the central nervous system (CNS). Finding biomarkers in the earliest stages of neurodegenerative diseases has become imperative, since, at the moment, there are no drugs that can reverse these pathological processes. Untargeted metabolomics analysis by liquid chromatography combined with SWATH-MS relative quantification is an emerging approach to search for potential biomarkers. In this chapter, we describe a method for untargeted metabolomics analysis of CSF samples that can also be used in parallel to a proteomics approach. The analysis is focused on the SWATH acquisition mode, where beyond precursor’s relative quantification, the information of the MS/MS relative quantification is also used to help in the search of new potential biomarkers.
Original languageEnglish
Title of host publicationMethods in molecular biology
PublisherHumana Press Inc.
Pages321-336
Number of pages16
ISBN (Electronic)9781493997060
ISBN (Print)9781493997053
DOIs
Publication statusPublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2044
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cerebrospinal fluid
  • Potential biomarkers
  • SWATH-MS relative quantification
  • Untargeted metabolomics

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