TY - JOUR
T1 - Use of tetramethylbenzidine for the spectrophotometric sequential injection determination of free chlorine in waters
AU - Mesquita, Raquel B. R.
AU - Noronha, M. Lúcia F. O. B.
AU - Pereira, Ana I. L.
AU - Santos, Arménia C. F.
AU - Torres, André F.
AU - Cerdà, Víctor
AU - Rangel, António O. S. S.
PY - 2007/5/15
Y1 - 2007/5/15
N2 - A sequential injection (SI) method was developed for the spectrophotometric determination of chlorine based on the reaction between tetramethylbenzidine (TMB) and free chlorine. The advantages resulting from the use of TMB are considerable: TMB is highly selective for chlorine, it enables a fairly low quantification limit and represents a less toxic alternative to reagents such as tolidine. The use of this reaction in SI adds other advantages as it enhances the degree of automation, minimisation of reagent consumption (6.8 μg TMB/assay) and low effluent production (2.5 mL/determination). The developed method allowed a quantification limit of 90 μg/L with a working range of 0.09-1.30 mg OCl-/L and a determination rate of 60 det./h. Based on these features, the system was applied to tap-water and surface water samples with no previous treatment required. The results obtained with the developed system were compared to the reference method, diethyl-p-phenylelediamine (DPD) colorimetric method, and proved not to be statistically different.
AB - A sequential injection (SI) method was developed for the spectrophotometric determination of chlorine based on the reaction between tetramethylbenzidine (TMB) and free chlorine. The advantages resulting from the use of TMB are considerable: TMB is highly selective for chlorine, it enables a fairly low quantification limit and represents a less toxic alternative to reagents such as tolidine. The use of this reaction in SI adds other advantages as it enhances the degree of automation, minimisation of reagent consumption (6.8 μg TMB/assay) and low effluent production (2.5 mL/determination). The developed method allowed a quantification limit of 90 μg/L with a working range of 0.09-1.30 mg OCl-/L and a determination rate of 60 det./h. Based on these features, the system was applied to tap-water and surface water samples with no previous treatment required. The results obtained with the developed system were compared to the reference method, diethyl-p-phenylelediamine (DPD) colorimetric method, and proved not to be statistically different.
KW - Free chlorine
KW - Sequential injection
KW - Spectrophotometry
KW - Tetramethylbenzidine
KW - Waters
UR - http://www.scopus.com/inward/record.url?scp=34247381053&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2007.01.010
DO - 10.1016/j.talanta.2007.01.010
M3 - Article
C2 - 19071743
AN - SCOPUS:34247381053
SN - 0039-9140
VL - 72
SP - 1186
EP - 1191
JO - Talanta
JF - Talanta
IS - 3
ER -