TY - JOUR
T1 - Utilization of glycerol during consecutive cycles of Lactobacillus reuteri fermentation under pressure
T2 - the impact on cell growth and fermentation profile
AU - Mota, Maria J.
AU - Lopes, Rita P.
AU - Sousa, Sérgio
AU - Gomes, Ana M.
AU - Lorenzo, Jose M.
AU - Barba, Francisco J.
AU - Delgadillo, Ivonne
AU - Saraiva, Jorge A.
PY - 2018/12
Y1 - 2018/12
N2 - Exposure of bacterial cells to sub-lethal high pressure (HP) during growth and fermentation may promote development of new adaptive features, with potential biotechnological interest. The present work evaluated the effect of consecutive fermentation cycles under HP on Lactobacillus reuteri growth and glycerol/glucose co-fermentation. At all conditions tested (0.1, 10 and 25 MPa), 1,3-propanediol production from glycerol was enhanced over the cycles. The highest titers, yields and productivities were achieved at 10 MPa. In addition, the HP-cycles promoted shifts in by-product formation (ethanol, acetate and lactate), with different profiles according to the pressure. Ratios between 1,3-propanediol:by-products increased throughout the cycles, especially at 10 MPa, indicating shifts in metabolic selectivity. Data regarding the effects of HP-cycles on protein and nucleic acid leakage suggested that, in some cases, L. reuteri changed membrane permeability as a possible adaptation to HP. Overall, this work confirms that HP may be an useful tool to stimulate production of 1,3-propanediol, as well as other biocompounds.
AB - Exposure of bacterial cells to sub-lethal high pressure (HP) during growth and fermentation may promote development of new adaptive features, with potential biotechnological interest. The present work evaluated the effect of consecutive fermentation cycles under HP on Lactobacillus reuteri growth and glycerol/glucose co-fermentation. At all conditions tested (0.1, 10 and 25 MPa), 1,3-propanediol production from glycerol was enhanced over the cycles. The highest titers, yields and productivities were achieved at 10 MPa. In addition, the HP-cycles promoted shifts in by-product formation (ethanol, acetate and lactate), with different profiles according to the pressure. Ratios between 1,3-propanediol:by-products increased throughout the cycles, especially at 10 MPa, indicating shifts in metabolic selectivity. Data regarding the effects of HP-cycles on protein and nucleic acid leakage suggested that, in some cases, L. reuteri changed membrane permeability as a possible adaptation to HP. Overall, this work confirms that HP may be an useful tool to stimulate production of 1,3-propanediol, as well as other biocompounds.
KW - Adaptation
KW - Fermentation
KW - Glycerol
KW - High pressure
UR - http://www.scopus.com/inward/record.url?scp=85053841479&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2018.08.034
DO - 10.1016/j.procbio.2018.08.034
M3 - Article
AN - SCOPUS:85053841479
VL - 75
SP - 39
EP - 48
JO - Process Biochemistry
JF - Process Biochemistry
SN - 0032-9592
ER -