Validation of a high performance liquid chromatography method for the stabilization of epigallocatechin gallate

Joana F. Fangueiro, Alexander Parra, Amélia M. Silva, Maria A. Egea, Eliana B. Souto*, Maria L. Garcia, Ana C. Calpena

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Epigallocatechin gallate (EGCG) is a green tea catechin with potential health benefits, such as anti-oxidant, anti-carcinogenic and anti-inflammatory effects. In general, EGCG is highly susceptible to degradation, therefore presenting stability problems. The present paper was focused on the study of EGCG stability in HEPES (N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid) medium regarding the pH dependency, storage temperature and in the presence of ascorbic acid a reducing agent. The evaluation of EGCG in HEPES buffer has demonstrated that this molecule is not able of maintaining its physicochemical properties and potential beneficial effects, since it is partially or completely degraded, depending on the EGCG concentration. The storage temperature of EGCG most suitable to maintain its structure was shown to be the lower values (4 or -20 °C). The pH 3.5 was able to provide greater stability than pH 7.4. However, the presence of a reducing agent (i.e., ascorbic acid) was shown to provide greater protection against degradation of EGCG. A validation method based on RP-HPLC with UV-vis detection was carried out for two media: water and a biocompatible physiological medium composed of Transcutol®P, ethanol and ascorbic acid. The quantification of EGCG for purposes, using pure EGCG, requires a validated HPLC method which could be possible to apply in pharmacokinetic and pharmacodynamics studies.

Original languageEnglish
Pages (from-to)181-190
Number of pages10
JournalInternational Journal of Pharmaceutics
Volume475
Issue number1-2
DOIs
Publication statusPublished - 20 Nov 2014
Externally publishedYes

Keywords

  • Catechins
  • Epigallocatechin gallate (EGCG)
  • Reverse phase high performance liquid chromatography (RP-HPLC)
  • Validation

Fingerprint

Dive into the research topics of 'Validation of a high performance liquid chromatography method for the stabilization of epigallocatechin gallate'. Together they form a unique fingerprint.

Cite this