TY - JOUR
T1 - Wool-associated proteolytic bacteria, isolated from Portuguese Merino breed
AU - Queiroga, A. Catarina
AU - Pintado, Manuela E.
AU - Malcata, F. Xavier
PY - 2013/1
Y1 - 2013/1
N2 - The main purpose of this study was to isolate and briefly characterize proteolytic bacteria from a poorly known habitat - raw wool. Fleece samples were accordingly collected from Merino raw wool - a Portuguese ewe breed, at three distinct areas of their body, from animals exhibiting no symptoms or signs of abnormalities; they were then subjected to enumeration and isolation of a total of 158 bacterial strains. Said isolates were screened for protease activity, using the spot technique, on Calcium Caseinate Agar containing 1% (w/v) skim milk. The 36 isolates displaying the highest protease activity underwent a more refined assessment of enzymatic performance - by examining their cell-free supernatant extracts, using casein as substrate. Two Bacillus isolates were eventually chosen owing to their highest proteolytic activities (24.6 and 15.9. U/mL), and identified using molecular biology tools.
AB - The main purpose of this study was to isolate and briefly characterize proteolytic bacteria from a poorly known habitat - raw wool. Fleece samples were accordingly collected from Merino raw wool - a Portuguese ewe breed, at three distinct areas of their body, from animals exhibiting no symptoms or signs of abnormalities; they were then subjected to enumeration and isolation of a total of 158 bacterial strains. Said isolates were screened for protease activity, using the spot technique, on Calcium Caseinate Agar containing 1% (w/v) skim milk. The 36 isolates displaying the highest protease activity underwent a more refined assessment of enzymatic performance - by examining their cell-free supernatant extracts, using casein as substrate. Two Bacillus isolates were eventually chosen owing to their highest proteolytic activities (24.6 and 15.9. U/mL), and identified using molecular biology tools.
KW - Caseinolytic activity
KW - Fleece
KW - Keratin
KW - Sheep
KW - Wool microflora
UR - http://www.scopus.com/inward/record.url?scp=84870322342&partnerID=8YFLogxK
U2 - 10.1016/j.smallrumres.2012.09.005
DO - 10.1016/j.smallrumres.2012.09.005
M3 - Article
AN - SCOPUS:84870322342
SN - 0921-4488
VL - 109
SP - 38
EP - 46
JO - Small Ruminant Research
JF - Small Ruminant Research
IS - 1
ER -