3D bioprinting using tissue-derived bioinks

Abstract

3D bioprinting allows for the detailed creation of tissue-like structures using bioinks made from biomaterials and cells. Decellularized extracellular matrix (dECM), extracted from natural tissues, provides bioactivity that helps cells survive and respond specifically to the tissue. In this thesis, we decellularized pig skin to create a hydrogel at a concentration of 2.5 mg.ml-1. We tested it as a potential bioink for extrusion-based bioprinting. To check if the decellularization process was successful, we conducted biochemical assays. These tests showed that the key components of the extracellular matrix (ECM) were mostly preserved. The hydrogel showed shear-thinning behavior and gelation suitable for printing. However, its structural integrity was limited to two layers, which indicated weak mechanical stability. L929 fibroblasts remained alive and showed continued spreading at 24 hours and 72 hours. These results highlight the potential of porcine dECM bioinks and emphasize the need for strategies, such as biocompatible crosslinking, to enhance long-term mechanical support.

Date of Award	18 Dec 2025
Original language	English
Awarding Institution	Universidade Católica Portuguesa
Supervisor	Estelle Palierse (Supervisor)