Biomarcadores salivares para as doenças cardiovasculares em pacientes portadores de periodontite crónica

  • Rafael José da Silva Cunha (Student)

Student thesis: Master's Thesis

Abstract

Introduction: Cardiovascular diseases are the leading cause of morbidity and mortality in the world, and the atherosclerosis is the most common cardiovascular disease. Recent studies have shown a relationship between chronic periodontitis and cardiovascular diseases. The biological plausibility for this association is based on the fact that the patients with periodontitis presenting increased levels of inflammation markers which are associated with the dysfunction of endothelium and the development of cardiovascular diseases. However, the mechanisms underlying these effects are not yet fully understood. Objectives: In this way, the aim of this work is clarify the biological and molecular mechanisms involved and to evaluate whether the salivary proteins may or may not be potential biomarkers differentiating the two conditions, as well as the association between chronic periodontitis and cardiovascular disease. Methods: To achieve these objectives, was made a literature search using the PubMed portal search tool, using the keywords, "cardiovascular diseases", "chronic periodontitis", "biomarkers", "proteomics", "salivary diagnosis". Subsequently, it was performed the manual annotation information concerning proteome of both diseases, with consequent creation of a database which enable update the OralCard. Finally, to be made an enrichment of the mechanisms involved in pathologies, we used the PANTHER bioinformatics tool. Results: The functional characterization of OralOme of diseases under study enabled the identification of changes in certain biological processes and molecular functions. The establishment of cardiovascular diseases proteoma allowed the addition of 25 new proteins to the OralCard data base. With this, it was possible, for the first time, to compile the information about cardiovascular diseases to this database. This research showed the existence of differences between the salivary protein quantification of the proteins common to both diseases. This knowledge will allow researchers to endorse studies on this area in order to identify differentiating biomarkers between these two conditions, as well as their association.
Date of Award26 Jul 2016
Original languagePortuguese
Awarding Institution
  • Universidade Católica Portuguesa
SupervisorMarlene Barros (Supervisor)

Keywords

  • Cardiovascular diseases
  • Chronic periodontitis
  • Biomarkers
  • Proteomics
  • Salivary diagnostics

Designation

  • Mestrado em Medicina Dentária

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