Caracterização do biofilme periodonto-patogénico numa amostra da população de pacientes com periodontite crónica avançada da UCP

  • Célia Coutinho Alves (Student)

Student thesis: Master's Thesis


Introduction: The evolution of severe chronic periodontitis is reliant on thepatients oral and periodontal health status due to resulting biological factors,hygiene and health related habits, genetical determinants and patients age.Despite this, the major factors that most influence the evolution of the periodontaldisease are the specific periodontal pathogens responsible for taking advantageof a debilitated periodontal status that enhances periodontal disease progression.The main objective was to study the periodontal pathogenic biofilm profile inindividuals with severe chronic periodontitis disease from the dental clinic of UCPby evaluating oral and periodontal status and by molecular detection ofperiodontal pathogens in salva and biofilm samples of healthy and periodontalpatients.Material and Methods: A sample of 29 patients was studied resulting in a controlgroup with 10 completely healthy patients and a test group with 19 patients withsevere chronic periodontitis. Three different data collection strategies wereemployed: sociodemographic data collection survey, clinical evaluation of oral andperiodontal status and saliva and biofilm sample collection.Results: The healthy status of the control group was confirmed and noperiodontal disease was reported. The same could not be said about the testgroup where severe chronic periodontitis was present on all samples. The DMFTindex average score for the control group was 7 ± 4,137 contrasting with the testgroup’s average DMFT index of 15,11 ± 9,11. Porphyromonas gingvalis andFilifactor alocis were only detected on the dental biofilm of periodontal sockets ofpatients with severe chronic periodontitis making it possible to get a non-clinical confirmation of it.Conclusion: It is possible to observe the installed periodontal disease in patientswith severe chronic periodontitis through the presence of specific periodontalbacteria in the biofilm of periodontal sockets of the test group, making the biofilmsample and analysis more reliable.
Date of Award2017
Original languagePortuguese
Awarding Institution
  • Universidade Católica Portuguesa
SupervisorCélia Coutinho Alves (Supervisor) & Maria José Correia (Co-Supervisor)


  • Oral health
  • Severe chronic periodontitis
  • Dental biofilm
  • Periodontal pathogens


  • Mestrado em Medicina Dentária

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