Criação da base de dados oralM associada à base OralOme

  • Carlos Eduardo Nogueira Sá (Student)

Student thesis: Master's Thesis


Introduction: Despite being a well-studied system from a microbiological point of view, the oral cavity presents a huge complexity of microenvironments and associated microbiota. This complexity has only recently been explored with the revolution in genomic technologies and techniques that allowed the identification of many, until recently, unkown microorganisms that could not be recognized in culture. The Human Microbiome Project (HMP) is presented as an example of the massive amount of data that can be generated by these techniques and that can be used on an extensive range of applications generating knowledge not only for the bacterial communities but also the processes in the oral cavity. Besides the HMP, molecular identification of microorganisms in various environments of the oral cavity either in health or in cases of oral or systemic pathology have increased in literature. These data are scattered and a database that gathers the published information with a research/analysis tool is extremely useful for researchers dedicated to the study of the oral cavity. Goals: To create a database with the microorganisms identified by genomic studies in samples from various environments of the oral cavity. To associate this database to the existing database OralOme. Materials and Methods: Bring together the information published in studies with identification of microorganisms by molecular techniques in various environments of the oral cavity in a database. The new database was designed to interact with the OralOme database allowing the exploration of microorganisms associated with the different environments of the oral cavity not only by name but also by the proteins produced by them. Results: The analysis of 111 articles published up to January 2014 showed 1399 species that represent 181 genera in the oral cavity. These values are above the current values y existing on the OralCard database that contains bacterial proteins. It also appears that the majority of typical species of the oral cavity (previously identified by cultivation techniques) is found in the oral cavity by molecular techniques. However, these new techniques detect other bacterial species cultured and often distinguish strains and clones are characteristic of the oral cavity.Conclusion: A database was created with the information of microbiome of the oral cavity, which is related to the OralOme database that provides information about the proteome of the oral cavity, creating a tool that contains the "total molecular evidence" of the oral cavity.
Date of Award9 Sept 2014
Original languagePortuguese
Awarding Institution
  • Universidade Católica Portuguesa
SupervisorMaria José Correia (Supervisor) & Joel Arrais (Co-Supervisor)


  • Human microbiome
  • Microhabitats
  • Genome
  • Database


  • Mestrado em Medicina Dentária

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