Eficácia da inactivação dos linfócitos nos pools de plaquetas após tratamento com Mirasol em comparação com a gama irradiação

Abstract

The transfusion of blood components is now an essential supportive therapy for the treatment and support in many medical and surgical situations. However, the transfer of leukocytes in transfusion of homologous blood may be associated with several risk of adverse effects such as transfusion associated - graft versus host disease (TA-GVHD), alloimmunization and febrile non-hemolytic transfusion reactions (FNHTR). For the prevention of TA-GVHD, gamma irradiation method is recommended by the food and drug administration, american association of blood bank and the council of europe, but this technology does not prevent alloimmunization nor FNHTR. In this context, new technologies has been developed to reduce pathogens treatment (PRT), such as the use of riboflavin (vitamin B2) that when exposed to ultraviolet light modifies the nucleic acids by inhibiting the replication of pathogens and leukocytes in blood products. Aims: This study compares the gamma irradiation with the inactivation method PRT in the inactivation of leukocytes in a pool, prepared in regional blood center in porto (Portugal) from automated atreus 3C. Materials and Methods: The T cells were obtained from concentrates leucoresiduais Atreus® 3C automated system (CaridianBCT, Belgium) and isolated by ficoll-histopaque method. One hiperpool was created with the four leucoresiduais concentrated and divided into three pools , the first got irradiated with 33Gy dose (Gammacell® 3000 Elite ®, MDS Nordion), the second with the method for inactivating Mirasol® PRT System and the third to control the process (no treatment). The samples were subjected to stimulation with phorbol myristate acetate, PMA (except the negative control), and measured the CD69 expression on T lymphocytes. Results: The average of CD69 on T-lymphocytes (não sei como se escreve) and CD3-positive of the irradiated sample was 6,72% whereas the inactivated samples by the PRT method was 0,29%. In negative control the average CD69 was 1,12% while the positive control was 96,54%. This study has shown that both the samples gamma irradiated and inactivated by PRT drastically decrease the CD69 expression. Conclusion: This study has shown that both the samples gamma irradiated and inactivated by PRT drastically decrease the CD69 expression.

Date of Award	Dec 2012
Original language	Portuguese
Awarding Institution	Universidade Católica Portuguesa
Supervisor	Salomé Maia (Supervisor) & Elísio Costa (Co-Supervisor)