Diabetes is a metabolic disorder characterized by a hyperglycemia. The increasing prevalence of diabetes and its associated complications imply a growing need for new strategies for early identification of individuals at risk. In general, the control of diabetes occurs through an invasive method that involves the measurement of plasma glucose. However, the assessment of salivary glucose begins to be well accepted in the scientific community and several studies were performed to ascertain the main advantages of this methodology. The complications associated with hyperglycemia are widely known, particularly in the health of the oral cavity with the development of periodontal disease. However, the relationship between glucose measured in saliva and its effects on the development of periodontitis is not well characterized and so far no study has been conducted in Portuguese diabetic population. With this project we aim to use a non-invasive method for glucose determination and to understand the effect of glucose in the oral cavity and its effect at periodontal disease. Therefore, three main objectives were defined for this study: 1. To establish a methodology for the determination of salivary glucose levels and the optimization of a technique that includes collection, storage and processing of the sample; 2. To evaluate the salivary glucose levels in a group of diagnosed T2DM subjects and to compare with healthy subjects (control group); 3. To assess the role of salivary hyperglucose in periodontal disease evolution. The salivary glucose levels were determined by using the glucose oxidation method and by applying a new adaptation to the D-Glycose GOD-POD method. Periodontal diagnosis of samples was based on periodontal clinical record in the University Dental Clinics of the Health Sciences Institute of the Portuguese Catholic University, in Viseu. In this study it was possible to optimize the assessment of the value of salivary glucose using a methodology for determining glucose levels in saliva, with the establishment of a new protocol – Standard Operating Protocol (SOP). Our results showed that the salivary glucose levels of diabetics were higher than the levels of non-diabetics. However, salivary glucose levels based on gender, age of the patient and duration of diabetes have not shown significant differences. Moreover, it was observed that in a hyperglucose situation there is a higher incidence of oral disease. When subjects were asked regarding the preference for invasive and non-invasive methodology the preference for the method of salivary glycose determination was notorious. In conclusion, with this project it was possible to implement a methodology to assess the salivary glucose in the University Dental Clinics of the Health Sciences Institute of the Portuguese Catholic University, in Viseu. Furthermore, with this methodology it was possible to observe an increase of the salivary glucose in diabetic subjects compared to control subjects.
Date of Award | 1 Aug 2016 |
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Original language | Portuguese |
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Awarding Institution | - Universidade Católica Portuguesa
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Supervisor | Joana Margarida Rosmaninho Salgado (Supervisor) & Tiago Marques (Co-Supervisor) |
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- Diabetes
- Saliva
- Hyperglycaemia
- Salivary glycose
- Periodontitis
- Mestrado em Medicina Dentária
Estudo piloto de avaliação da glicose salivar em seniores
Miranda, V. (Student). 1 Aug 2016
Student thesis: Master's Thesis