Identificação de biomarcadores salivares de doença periodontal em pacientes com diabetes Mellitus tipo 2

Abstract

Introduction: Periodontitis (inflammatory disease induced by microorganisms that affect the tooth supporting structures) and Type 2 Diabetes Mellitus (endocrine pathology associated with hyperglycemia) are chronic diseases that are highly prevalent in modern society. The protein expression alterations associated with the pathophysiology of the disease are likely to be assessed in saliva, enabling the noninvasive diagnosis. Objectives: To quantify, in saliva of patients with periodontal disease and type 2 diabetes, potential biomarkers proposed by in silico studies based on the relative information of the markers expression profile in patients with only one disease, chronic periodontitis or type 2 diabetes. Materials and Methods: Collection and processing of saliva in patients with chronic periodontitis, with and without type 2 diabetes, according to the SalivaTec protocol from Universidade Católica Portuguesa. Storage of samples following the guidelines of Biobanco from Instituto de Medicina Molecular da Universidade de Lisboa. Evaluation of chemokine (CC motif) ligand 13 (CCL13) (Q99616) and chemokine (CC motif) ligand 3 (CCL3) (P10147), two chemokines indicators of the Th subpopulations of lymphocytes and macrophages present in chronic inflammation. Quantification was performed by multiplex technology. Results: Saliva samples from 22 healthy patients, 14 patients suffering from chronic periodontitis and 28 patients with chronic periodontitis and type 2 diabetes were collected. Although statistically significant (p <0.05) differences were found among the three study groups for CCL3 and the CCL13, only comparisons between the group of healthy and the group of patients with periodontal disease and type 2 diabetes were significant for both chemokines. Conclusions: The results of the present work indicate that the protein expression profile in multi-compromised patients is distinct from the expression values of the same chemokines in patients with only one disease, type 2 diabetes or periodontal disease, demonstrating the need of such studies for the validation of biomarkers.

Date of Award	29 Jul 2015
Original language	Portuguese
Awarding Institution	Universidade Católica Portuguesa
Supervisor	Marlene Barros (Supervisor)