Imunofenotipagem por citometria de fluxo

Abstract

As final work of the Master Degree in Clinical Analysis and Public Health - Specialty of Hematology and Imunohemoterapy held a traineeship. This was occured between the period February to May 2011 and it happened in Immunology Laboratory in the Pathology Service in the Hospital Center of São João, EPE. During this period, followed the routine of Flow Cytometry laboratory, from the reception of the samples to the elaboration of its final report. This, essentially devoted to the study of blood-oncological pathologies, performing immunophenotyping of samples, taking into account the information provided or diagnosis. The samples studied included peripheral blood, bone marrow aspirates and other biological fluids such as pleural, peritoneal, pericardial and cerebrospinal fluid and aspiration biopsies of lymph nodes and masses. It carried even the quantification of CD34 + cells in peripheral blood and apheresis products in patients in mobilization to perform autotransplantation. Apart from the study of blood-oncological pathologies, also carries out the quantitation of lymphocyte populations in patients with HIV/AIDS. In the selection of the phenotypic profile, are used panels of monoclonal antibodies, as defined by the European Project: "Euroflow" 52. These are used according to clinical information provided. Nowadays, it is known that the application of Flow Cytometry has shown an excellent complementary means of diagnosis.

Date of Award	2013
Original language	Portuguese
Awarding Institution	Universidade Católica Portuguesa
Supervisor	Maria Cristina Fragoso Marques (Supervisor) & João Tiago Guimarães (Supervisor)