Influência dos factores obstétricos e do volume inicial de sangue do cordão umbilical na contagem de células CD34

  • Iolanda Margarida de Oliveira Grenha (Student)

Student thesis: Master's Thesis

Abstract

Placenta´s blood contains hematopoietic stem cells capable of reconstituting long-term hematopoiesis when transplanted after myeloablative conditioning. Some studies have demonstrated that the quality of the cord blood units can be influenced by many obstetric factors and by those who make the harvest. The CD34+ cell count, total number of nucleated cells and cord blood´s volume are determining variables as acceptance criteria for bank of umbilical cord cells. The aim of this work is to evaluate the influence of obstetrics factors and the initial cord blood´s volume in CD34+ cell count. This study used data from 179 samples of umbilical cord blood cryopreserved in LusoCord - North Histocompatibility Centre between April and October 2010. The harvest of umbilical cord blood was performed by two methods: in utero or ex utero. The processing of these samples was constituted: by monitoring the gathered volume, WBC count and total nucleated cell (TNC) count, volume reduction before cryopreservation, CD34+ cell count by flow cytometry following the ISHAGE protocol and the measurement of the viability resorting to the nucleic acid marker 7-aminoactinomycin D. We found a significant statistically correlation between the initial volume of the unit and between the final TNC value. There was also a correlation between the total number of CD34+ cells and maternal age. There was a significant statistically correlation between the weight of the babies and the initial volume. It can be concluded that obstetric factors and initial volume interfere with CD34+ cells count. A higher initial volume is associated with a greater TNC and greater number of CD34+ cells. CD34+ cell count is higher in younger mothers. The umbilical cord blood of heavier infants resulted in a higher initial volume.
Date of AwardSept 2012
Original languagePortuguese
Awarding Institution
  • Universidade Católica Portuguesa
SupervisorSandra Cristina Ribeiro Tafulo, (Supervisor) & Elísio Costa (Co-Supervisor)

Designation

  • Mestrado em Análises Clínicas e Saúde Pública

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