Interactómica da cavidade oral em doença periodontal

  • Mafalda Ribeiro Cardoso de Barros (Student)

Student thesis: Master's Thesis

Abstract

Introduction: Proteomics is the field of biotechnology that analysis the entire set of proteins of a biological sample, particularly their structures and functions. In the oral cavity there are a wide variety of human proteins, which in the case of periodontal disese may be altered due to the interaction with the pathogen-produced proteins. Bioinformatic tools have an important role in providing and analysing data that allow the establishment of the host-microbe interactome. Objective: In silico analysis through bioinformatic tools; Establishment of the interactome during cronic peridonditis; Identification of the alterations in biological processes of the host oral cavity induced by the presence of microorganisms; Identication os key-proteins involved in periodontal disease. Materials and Methods: Three bioinformnatic tools were used: OralCard, Oralint and Cytoscape. OralCard was used to list human and microbial proteins present in the oral cavity of patients with cronic periodonditis. Oralint was used to verify protein-protein interactions and Cytoscape to represent the complex network of interactions. Functional annotation was performed with AgBase. Results and Discussion: A total of 1071 proteins were catalogued in the OralCard. From these, 1039 were human and 31 microbial. The two most relevant biological processes selected were: Extracellular matrix organization and Immune system process, thus reducing the sample number to 50 human and 7 microbial proteins. The majority of the human proteins was up-regulated and, through the interaction, may contribute do disease progression, infection control or for the re-establishment of the host equilibrium. Conclusions and Future Works: In this study a set of proteins was identified which might be important for the development and control of cronic periodonditis. More effective studies will be needed in the future to better clarify the function of each protein during the pathology.
Date of Award9 Sept 2014
Original languagePortuguese
Awarding Institution
  • Universidade Católica Portuguesa
SupervisorNuno Rosa (Supervisor) & Maria José Correia (Co-Supervisor)

Keywords

  • Proteins of the oral cavity
  • OralOme
  • Protein-protein interactions
  • Interatomic
  • Periodontal disease

Designation

  • Mestrado em Medicina Dentária

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