MiRNAs salivares como biomarcadores da diabetes Mellitus tipo II (DMTH)

Abstract

Objectives: Study the potential of miRNAs salivary as biomarkers of T2DM, by the systematic review of the evidence available on changes in the expression of miRNA associated with T2DM and identify the miRNAs which have their expression altered in T2DM and have been detected in saliva, making them promising candidates to be used as salivary biomarkers in T2DM. Methods: Systematic search in PubMed and Web of Science databases to identify studies where miRNAs have been quantified in patients with T2DM and to build a database with the annotation of the results of these articles. Statistical analysis of the global results to determine which miRNAs can become T2DM biomarkers. Check if some of the identified miRNAs have been identified in oral fluids and / or which can be found in these fluids. Results: Were found 776 unduplicated articles mentioning miRNAs as biomarkers for T2DM, of which 115 were selected but only 31 included in the results because they fulfilled the inclusion criteria. From the analysis of these articles, 53 miRNAs were found to be altered in samples from diabetics vs. samples from patients with normal glucose tolerance, 17 are changed when comparing patients with altered glucose tolerance (prediabetes) vs. patients with normal glucose tolerance and 13 are altered when comparing diabetics vs. patients with altered glucose tolerance (prediabetes). Among these three group comparisons, 49 miRNAs that we propose as potential biomarkers of T2DM exist. These 41 are in saliva, of which the best’s potentials biomarkers are miRNA 125b; miRNA 519c-3p; miRNA 519d-3p; miRNA 802; miRNA 486-5p; miRNA 136-5p, miRNA 369-3p, miRNA 411-5p, miRNA 487a-3p, miRNA 487b-3p, miRNA 655-3p, miRNA 656-3p, miRNA 432-5p, miRNA 593-3p and miRNA 128, because they are involved in several pathophysiological processes of T2DM. Conclusion: At the moment, there is no consensus about having exclusive miRNAs for Diabetes Mellitus Type II. However, the bioinformatics analysis done in this work allows the proposal of a group of miRNAs that should be investigated as potential biomarkers of T2DM

Date of Award	9 Dec 2016
Original language	Portuguese
Awarding Institution	Universidade Católica Portuguesa
Supervisor	Maria José Correia (Supervisor) & Luís Santos (Co-Supervisor)