O proteoma do microbioma oral humano

: que funções exercem as proteínas microbianas?

Abstract

In the OralOme database, available through the OralCard interface, information resulting from proteomics studies of oral tissues and in vitro studies is gathered and updated. Besides the host proteins, proteins produced by the oral microbiome are includes ant it is possible to know which are produced by each genre and / or species. This bioinformatics tool facilitates the development of new diagnostics, prognostics and the search for new therapeutic agents. Objective: Using the data deposited in the OralCard relative to the proteins produced by the oral microbiome, a critical analysis of the functional role performed by the proteins of bacterial origin was conducted. Emphasis was given to proteins involved in the pathogenesis biological process. Materials and methods: The oral microbiome proteins were analyzed as to the biological processes in which they participate. A functional analysis of the proteins classified with the GO "pathogenesis" was performed using public databases. Results: Of the 10 428 bacterial proteins analyzed, 298 were classified with the GO "pathogenesis". Of these, only 44 are characterized at the protein level and the remaining, have evidence at the gene level. The analysis revealed that only 11 of the 298 were identified in samples from the oral cavity and the remaining identified in vitro. The specific mechanisms of virulence with more number of associated proteins are the survival of the organism within the host cell and it’s lysis. Conclusion: The specific mechanisms of the pathogenesis for most oral microbiome proteins are well described. However, almost all proteins have been described in vitro studies. Therefore, it would be desirable that future studies, reveal the existence of these proteins in saliva samples from patients.

Date of Award	5 Sept 2014
Original language	Portuguese
Awarding Institution	Universidade Católica Portuguesa
Supervisor	Maria José Correia (Supervisor) & Marlene Barros (Co-Supervisor)