Utilização do método ATP bioluminescência na avaliação da eficácia da limpeza e desinfeção de superfícies em cuidados de saúde primários

  • Helena Marina Massena Ferreira (Student)

Student thesis: Master's Thesis


The risk of acquiring infections due to care provided at health centers, is now becoming a concern. In the treatment rooms during the execution of procedures, work surfaces may be contaminated and constitute themselves as potential reservoirs or sources of pathogenic and multiresistant microorganisms. The aim of this study was to verify the adequacy of the ATP bioluminescence method in evaluating the effectiveness of cleaning and disinfection of work surfaces in primary health care. We conducted an observational, longitudinal, prospective and analytical study. The ATP bioluminescence method proved to be easy to execute, deliver immediate and measurable results (in URL). Based on the values of 66 measurements taken after the cleaning and disinfection of the bench and couch in a treatment room, was established the benchmark of 144 URL, valid for the luminometer used. We evaluated the benches and couches of 32 treatment rooms in five health centers in the Algarve region. We compared the results obtained by visual and ATP bioluminescence methods, before and after cleaning and disinfecting these surfaces. Using the method of Bioluminescence was detected a higher percentage of “faults” (not acceptable) than by visual observation (dirty), which was more evident in the post-cleaning. Applying the McNemar test ( = 0,10; gl = 1), it was found that the visual and ATP bioluminescence methods produce results without significant differences before cleaning and disinfection of surfaces and with significant differences after. In conclusion, the visual inspection proved to be a method of subjective assessment of the effectiveness of the cleaning and disinfection of work surfaces, while the method ATP bioluminescence was found to be a good indicator of the evaluation.
Date of Award2012
Original languagePortuguese
Awarding Institution
  • Universidade Católica Portuguesa
SupervisorEugénia Maria Dores Maia Ferreira Castela (Supervisor)


  • Primary health care
  • Infection associated with health care
  • Environmental surfaces
  • Cleaning and disinfection
  • ATP bioluminescence method
  • Método ATP bioluminescência


  • Mestrado em Infeção em Cuidados de Saúde

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