A spectrophotometric assay for robust viability testing of seed batches using 2,3,5-triphenyl tetrazolium chloride: Using Hordeum vulgare L. as a model

Laura Lopez Del Egido*, David Navarro-Miró, Victor Martinez-Heredia, Peter E. Toorop, Pietro P.M. Iannetta

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

34 Citações (Scopus)

Resumo

A comparative analysis was carried out of published methods to assess seed viability using 2,3,5-triphenyltetrazolium chloride (TTC) based assays of seed batches. The tests were carried out on seeds of barley (Hordeum vulgare cv. Optic) as a model. We established that 10%[w/v] trichloroacetic acid (TCA)/methanol is superior to the acetone and methanol-only based methods: allowing the highest recovery of formazan and the lowest background optical density (OD) readings, across seed lots comprising different ratios of viable and dead seeds. The method allowed a linear-model to accurately capture the statistically significant relationship between the quantity of formazan that could be extracted using the method we developed and the seed temperature-response, and seed viability as a function of artificially aged seed lots. Other quality control steps are defined to help ensure the assay is robust and these are reported in a Standard Operating Procedure.

Idioma originalEnglish
Número do artigo747
RevistaFrontiers in Plant Science
Volume8
DOIs
Estado da publicaçãoPublicado - 16 mai. 2017
Publicado externamenteSim

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