Acetylation drives hepatocyte nuclear factor 1β stability by blocking proteasome-mediated degradation

Filipa Lopes-Coelho, Fernanda Silva, Ana Hipólito, Bruno A. Cardoso, Jacinta Serpa

Resultado de pesquisarevisão de pares

3 Citações (Scopus)

Resumo

Hepatocyte nuclear factor 1β (HNF1β) is mostly expressed in the liver, but is also expressed in other organs, like kidney, pancreas and genitourinary tract. In fact, HNF1β, a member of the superfamily of homeodomain-containing transcription factors, has been described as a hallmark in clear cell carcinomas. However, its role as an oncogene or as tumor suppressor gene remains controversial. Here, we disclose a mechanism of HNF1β stabilization and degradation, using human HNF1β-expressing cell lines of ovarian clear cell carcinoma (ES2), hepatocellular carcinoma (HEPG2), and normal immortalized kidney tubular cells (HK2). We show that increased levels of HNF1β is concomitant with an increase in the acetylation load and protein stabilization by interfering with the ubiquitin-proteasome degradation system. This study reinforces that acetylation, besides their role in regulating chromatin conformation and gene expression, could also act in the action, turnover and stability of proteins essential for the survival and progression of certain cancer types.
Idioma originalEnglish
Páginas (de-até)9337-9344
Número de páginas8
RevistaJournal of Cellular Biochemistry
Volume120
Número de emissão6
DOIs
Estado da publicaçãoPublicado - jun 2019
Publicado externamenteSim

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