Adsorption of proteins onto non-soluble polysaccharides matrixes: a friendly strategy to isolate enzymes with potential application for downstream processes

Nadia Woitovich Valetti, M. Emilia Brassesco, Guillermo Alfredo Pico

Resultado de pesquisarevisão de pares

Resumo

Polysaccharides with electrically charged groups, polyelectrolytes (PE), have the capacity to form hydrogels under different experimental conditions, which act as ionic exchanges with high affinity to adsorb proteins. Presented in this chapter is a description of the current state of the technic on the use of these matrixes. Alginate, carrageenan, chitosan, pectin, etc. are the most used PE to make beds with the capacity of proteins adsorption. The presence of electrically charged residual groups in these PE allows their use as beds for the ion exchange chromatography in stirred tank of packed bed or expended bed. We demonstrated the adsorption of lysozyme, a model protein positively charged, onto Alginate-Guar gum matrixes cross-linked with epichlorohydrin (negatively charged). Their physical characterization, equilibrium isotherms and adsorption kinetics were carried out. Successive cycles of adsorption-washing-elution were performed. The results demonstrate the reversibility of the process and the capacity of this enzyme purification method.
Idioma originalEnglish
Título da publicação do anfitriãoApplications of adsorption and ion exchange chromatography in waste water treatment
EditoresInamuddin , Amir Al-Ahmed
Capítulo4
Páginas84-106
Número de páginas23
ISBN (eletrónico)9781945291333
DOIs
Estado da publicaçãoPublished - 25 abr 2017
Publicado externamenteSim

Série de publicação

NomeMaterials Research Foundations
Volume15

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