Anti-viral photodynamic inactivation of T4-like bacteriophage as a mammalian virus model in blood

Patrícia Santos, Ana T. P. C. Gomes, Leandro M. O. Lourenço*, Maria A. F. Faustino, Maria G. P. M. S. Neves, Adelaide Almeida*

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

Resumo

The laboratorial available methods applied in plasma disinfection can induce damage in other blood components. Antimicrobial photodynamic therapy (aPDT) represents a promising approach and is approved for plasma and platelet disinfection using non-porphyrinic photosensitizers (PSs), such as methylene blue (MB). In this study, the photodynamic action of three cationic porphyrins (Tri-Py(+)-Me, Tetra-Py(+)-Me and Tetra-S-Py(+)-Me) towards viruses was evaluated under white light irradiation at an irradiance of 25 and 150 mW·cm−2, and the results were compared with the efficacy of the approved MB. None of the PSs caused hemolysis at the isotonic conditions, using a T4-like phage as a model of mammalian viruses. All porphyrins were more effective than MB in the photoinactivation of the T4-like phage in plasma. Moreover, the most efficient PS promoted a moderate inactivation rate of the T4-like phage in whole blood. Nevertheless, these porphyrins, such as MB, can be considered promising and safe PSs to photoinactivate viruses in blood plasma.
Idioma originalEnglish
Número do artigo11548
Número de páginas16
RevistaInternational Journal of Molecular Sciences
Volume23
Número de emissão19
DOIs
Estado da publicaçãoPublicado - 30 set 2022
Publicado externamenteSim

Impressão digital

Mergulhe nos tópicos de investigação de “Anti-viral photodynamic inactivation of T4-like bacteriophage as a mammalian virus model in blood“. Em conjunto formam uma impressão digital única.

Citação