Antilisterial active compound from lactic acid bacteria present on fresh iceberg lettuce

Pediococcus pentosaceus DT016, a bacteriocin producing strain, was isolated from fresh lettuce. A protein with antilisterial activity (bacDT016), between 11 to 17 kDa, was identified and characterized as the bioactive substance from the LAB culture. Highest bacteriocin production was noted after 15 h of growth. Antibacterial activity was affected by some enzymes and detergents, as well as by temperatures equal or above 80 °C. DT016 strain contains an 1110 bp DNA fragment with homology to pediocin AcH/PA-1.