TY - JOUR
T1 - Biochemical characterization of SFC-1, a class A carbapenem-hydrolyzing β-lactamase
AU - Fonseca, Fátima
AU - Sarmento, Ana Cristina
AU - Henriques, Isabel
AU - Samyn, Bart
AU - Van Beeumen, Jozef
AU - Domingues, Pedro
AU - Domingues, Maria Rosário
AU - Saavedra, Maria José
AU - Correia, António
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2007/12
Y1 - 2007/12
N2 - The carbapenem-hydrolyzing β-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrolyzes penicillins, cephalosporins, aztreonam, and carbapenems and is inhibited by clavulanic acid, sulbactam, and tazobactam.
AB - The carbapenem-hydrolyzing β-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrolyzes penicillins, cephalosporins, aztreonam, and carbapenems and is inhibited by clavulanic acid, sulbactam, and tazobactam.
UR - http://www.scopus.com/inward/record.url?scp=36749039667&partnerID=8YFLogxK
U2 - 10.1128/AAC.00491-07
DO - 10.1128/AAC.00491-07
M3 - Article
C2 - 17875998
AN - SCOPUS:36749039667
SN - 0066-4804
VL - 51
SP - 4512
EP - 4514
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 12
ER -