Biodegradation and Metabolic Pathway of 17β-Estradiol by Rhodococcus sp. ED55

Irina S. Moreira*, Sapia Murgolo, Giuseppe Mascolo, Paula M. L. Castro

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

Resumo

Endocrine disrupting compounds (EDCs) in the environment are considered a motif of concern, due to the widespread occurrence and potential adverse ecological and human health effects. The natural estrogen, 17β-estradiol (E2), is frequently detected in receiving water bodies after not being efficiently removed in conventional wastewater treatment plants (WWTPs), promoting a negative impact for both the aquatic ecosystem and human health. In this study, the biodegradation of E2 by Rhodococcus sp. ED55, a bacterial strain isolated from sediments of a discharge point of WWTP in Coloane, Macau, was investigated. Rhodococcus sp. ED55 was able to completely degrade 5 mg/L of E2 in 4 h in a synthetic medium. A similar degradation pattern was observed when the bacterial strain was used in wastewater collected from a WWTP, where a significant improvement in the degradation of the compound occurred. The detection and identification of 17 metabolites was achieved by means of UPLC/ESI/HRMS, which proposed a degradation pathway of E2. The acute test with luminescent marine bacterium Aliivibrio fischeri revealed the elimination of the toxicity of the treated effluent and the standardized yeast estrogenic (S-YES) assay with the recombinant strain of Saccharomyces cerevisiae revealed a decrease in the estrogenic activity of wastewater samples after biodegradation.

Idioma originalEnglish
Número do artigo6181
Número de páginas16
RevistaInternational Journal of Molecular Sciences
Volume23
Número de emissão11
DOIs
Estado da publicaçãoPublished - 30 mai 2022

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