Bioluminescent models to evaluate the efficiency of light-based antibacterial approaches

Ana T. P. C. Gomes, Maria A. F. Faustino, Maria G. P. M. S. Neves, Adelaide Almeida*

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares


The emergence of microbial resistance to antimicrobials among several common pathogenic microbial strains is an increasing problem worldwide. Thus, it is urgent to develop not only new antimicrobial therapeutics to fight microbial infections, but also new effective, rapid, and inexpensive methods to monitor the efficacy of these new therapeutics. Antimicrobial photodynamic therapy (aPDT) and antimicrobial blue light (aBL) therapy are receiving considerable attention for their antimicrobial potential and represent realistic alternatives to antibiotics. To monitor the photoinactivation process provided by aPDT and aBL, faster and more effective methods are required instead of laborious conventional plating and overnight incubation procedures. Bioluminescent microbial models are very interesting in this context. Light emission from bioluminescent microorganisms is a highly sensitive indication of their metabolic activity and can be used to monitor, in real time, the effects of antimicrobial agents and therapeutics. This chapter reviews the efforts of the scientific community concerning the development of in vitro, ex vivo, and in vivo bioluminescent bacterial models and their potential to evaluate the efficiency of aPDT and aBL in the inactivation of bacteria.
Idioma originalEnglish
Título da publicação do anfitriãoMethods in molecular biology
EditoresMans Broekgaarden, Hong Zhang, Mladen Korbelik, Michael R. Hamblin, Michal Heger
EditoraHumana Press Inc.
Número de páginas39
ISBN (eletrónico)9781071620991
ISBN (impresso)9781071620984
Estado da publicaçãoPublicado - 2022
Publicado externamenteSim

Série de publicação

NomeMethods in Molecular Biology
ISSN (impresso)1064-3745
ISSN (eletrónico)1940-6029

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