Biopolymer from water kefir as a potential clean-label ingredient for health applications: evaluation of new properties

Monalisa de Alencar Lucena; Igor Frederico da Silveira Ramos; Maurycyo Silva Geronço; Ricardo de Araújo; Francisco Lopes da Silva  Filho; Luís Manuel Lopes Rodrigues da Silva; Rayran Walter Ramos de Sousa; Paulo Michel Pinheiro Ferreira; Josy Anteveli Osajima; Edson Cavalcanti Silva-Filho; Márcia dos Santos Rizzo; Alessandra Braga Ribeiro; Marcilia Pinheiro da Costa

doi:10.3390/molecules27123895

Biopolymer from water kefir as a potential clean-label ingredient for health applications: evaluation of new properties

Monalisa de Alencar Lucena, Igor Frederico da Silveira Ramos, Maurycyo Silva Geronço, Ricardo de Araújo, Francisco Lopes da Silva Filho, Luís Manuel Lopes Rodrigues da Silva, Rayran Walter Ramos de Sousa, Paulo Michel Pinheiro Ferreira, Josy Anteveli Osajima, Edson Cavalcanti Silva-Filho, Márcia dos Santos Rizzo, Alessandra Braga Ribeiro^*, Marcilia Pinheiro da Costa

^*Autor correspondente para este trabalho

Resultado de pesquisa › revisão de pares

2 Transferências (Pure)

Resumo

The present work aimed to characterize the exopolysaccharide obtained from water kefir grains (EPSwk), a symbiotic association of probiotic microorganisms. New findings of the technological, mechanical, and biological properties of the sample were studied. The EPSwk polymer presented an Mw of 6.35 × 105 Da. The biopolymer also showed microcrystalline structure and characteristic thermal stability with maximum thermal degradation at 250 °C. The analysis of the monosaccharides of the EPSwk by gas chromatography demonstrated that the material is composed of glucose units (98 mol%). Additionally, EPSwk exhibited excellent emulsifying properties, film-forming ability, a low photodegradation rate (3.8%), and good mucoadhesive properties (adhesion Fmax of 1.065 N). EPSwk presented cytocompatibility and antibacterial activity against Escherichia coli and Staphylococcus aureus. The results of this study expand the potential application of the exopolysaccharide from water kefir as a potential clean-label raw material for pharmaceutical, biomedical, and cosmetic applications.

Idioma original	English
Número do artigo	3895
Número de páginas	25
Revista	Molecules
Volume	27
Número de emissão	12
DOIs	https://doi.org/10.3390/molecules27123895
Estado da publicação	Publicado - 17 jun 2022

Acesso ao documento

10.3390/molecules27123895Licença:

45517360Final published version, 3,16 MBLicença:

http://hdl.handle.net/10400.14/38119Licença:

Outros arquivos e links

Link to publication in Scopus

1 Ativos

CBQF Multianual Funding: Centro de Biotecnologia e Química Fina
Cortez, J., Pintado, M. M., Morais, A. M. M. B., Ribeiro, A. B., Ferreira, A. C., Castro, P., Villamor, A. L., Oliveira, A. L., Oliveira, A. L. S., Amaro, A. L., Paulo, A., Gomes, A. M., Marques, A., Carvalho, A. P., Monforte, A. R., Madureira, A. R., Oliveira, A. S., Ferreira, A. S., Rangel, A. O. S. S., Oliveira, C., Cálix, C., Pereira, C. F., Santos, C. S. D., Amorim, C. L., Oliveira, C., Manaia, C., Silva, C. L. M., Monteiro, C. M., Machado, D., Campos, D., Oliveira, D., Valente, D., Cardoso, E., Costa, E. M. A. D., Pinto, E., Alexandre, E., Carsanba, E., Coscueta, E., Raposo, M. F. D. J., Tavaria, F., Voss, G. B., Moreira, H., Campos, I., Tomada, I., Moreira, I. S., Vaz-Moreira, I., Fundo, J. F., Barbosa, J., Pereira, J. O., Durão, J. R. D. O., Costa, J. R., Fernandes, J. C., Ferreira, J., Silva, J. A., Couto, J. A., Oliveira, L., Pimentel, L., Rodríguez-Alcalá, L. M., Hogg, C., Poças, M. D. F., Miller, F. A., Brassesco, M. E., Mota, I., Ramos, I. N., Vasconcelos, I., Monteiro, M. J., Amorim, M., Roriz, M., Silva, M., Correia, M., Silva, M. N. D., Alves, M., Vasconcelos, M., Ramos, O. L., Costa, P., Gibbs, P., Teixeira, P., Ramos, P., Rodrigues, P. M., Carvalho, P., Mesquita, R., Barros, R., Morais, R. M. S. C., Magalhães, R. M. B. D. S., Borges, S. C. F., Correia, S., Silva, S. B. D., Silva, S., Moreira, S. A. M., Pedrosa, S., Pereira, S., Vidigal, S., Pedro, T., Ribeiro, T., Deuchande, T., Brandão, T. R. S., Resende, T., Hogg, T., Ferreira, V., Viseras, A. D., Soares, A. M. S., Oliveira, A. S., Uribe, B., Pais-Vieira, C., Ferreira, C., Sousa, C. C. S., Kumla, D., Carsanba, E., Lopes, G. L. L., Ribeiro, I. M. M. V. P., Cruz, I. B. D., Barbosa, J. C., Oliveira, L., Mendes, M. A. R., Boas, A. V., Cassoni, A. C., Lopes, A., Pintado, A. I. E., Sousa, A. P., Faustino, A., Salsinha, A. S., Couto, A. T., Macieira, A., Horta, B. M., Silva, C. M. E., Rodrigues, C. M., Bernardes, B. G., Silva, B. I. Q. L. D., Costa, C., Costa, C. M., Serafim, C., Santos, D., Antunes, F., Vieira, E., Ferreira, H., Moreira, I. A., Vieira, I. R. D. F., Rocha, J., Henggeler , J., Miranda, J., Oliveira, M., Lopes, M. T. B. V., Silva, N., Carvalho, N., Costa, P., Nova, P., Sousa, P., Silva, P., Gómez-García, R., Freixo, R., Marçal, S., Cunha, S. A., Sousa, S., Pereira, S. A., Ghalamara, S., Vilela, T., Lopes, A., Lopes, A. I. R., Linhares, A. L. S., Boas, A. M. M. V., Teixeira, A. M. R., Mendes, A. R. M., Oliveira, A. S., Sousa, A. S. D. S., Sousa, A. S. D. S., Martins, A. S. P. D. P., Veiga, A., Bernardes, B. G., Silva, B. I. Q. L. D., Fernandes, A. S., Miranda, C., Dias, C. L. A., Neto, C. C., Castro, C. M. O., Oliveira, D. F., Rodrigues, D., Magalhães, D. D. S., Almeida, D., Castro, D., Costa, E. C. L. D., Darú, F. A., Bastos, F., Teixeira, F. D. S., Ferreira, F., Fortunato, G., Araújo-Rodrigues, H., Osorio Perez, J. R., Silveira, J. F., Silva, J. A., Soares, J. C., Carvalho, L. C., Castro, L. M. G., Machado, M., Carvalho, M. J., Nazareth, M., Veiga, M., Coelho, M., Carvalho, M. I. P. D., Mendes, M., Ramos, M. A. M., Silva, T., Resende, T., Fontes, A. L., Neves, D. I. S. C. D., Cachetas, D. M., Silva, G. A. D. R. & Morais, R. M. S. C.
1/01/20 → 31/12/23
Projeto

Citação

Lucena, M. D. A., Ramos, I. F. D. S., Geronço, M. S., Araújo, R. D., Filho, F. L. D. S., Silva, L. M. L. R. D., Sousa, R. W. R. D., Ferreira, P. M. P., Osajima, J. A., Silva-Filho, E. C., Rizzo, M. D. S., Ribeiro, A. B., & Costa, M. P. D. (2022). Biopolymer from water kefir as a potential clean-label ingredient for health applications: evaluation of new properties. Molecules, 27(12), [3895]. https://doi.org/10.3390/molecules27123895

@article{6b3015e4e8784aae8f386c4dbde1e01c,

title = "Biopolymer from water kefir as a potential clean-label ingredient for health applications: evaluation of new properties",

abstract = "The present work aimed to characterize the exopolysaccharide obtained from water kefir grains (EPSwk), a symbiotic association of probiotic microorganisms. New findings of the technological, mechanical, and biological properties of the sample were studied. The EPSwk polymer presented an Mw of 6.35 × 105 Da. The biopolymer also showed microcrystalline structure and characteristic thermal stability with maximum thermal degradation at 250 °C. The analysis of the monosaccharides of the EPSwk by gas chromatography demonstrated that the material is composed of glucose units (98 mol%). Additionally, EPSwk exhibited excellent emulsifying properties, film-forming ability, a low photodegradation rate (3.8%), and good mucoadhesive properties (adhesion Fmax of 1.065 N). EPSwk presented cytocompatibility and antibacterial activity against Escherichia coli and Staphylococcus aureus. The results of this study expand the potential application of the exopolysaccharide from water kefir as a potential clean-label raw material for pharmaceutical, biomedical, and cosmetic applications.",

keywords = "Antimicrobial activity, Biopolymer, Dextran, Mucoadhesiveness, Photostability, Polysaccharide",

author = "Lucena, {Monalisa de Alencar} and Ramos, {Igor Frederico da Silveira} and Geron{\c c}o, {Maurycyo Silva} and Ara{\'u}jo, {Ricardo de} and Filho, {Francisco Lopes da Silva} and Silva, {Lu{\'i}s Manuel Lopes Rodrigues da} and Sousa, {Rayran Walter Ramos de} and Ferreira, {Paulo Michel Pinheiro} and Osajima, {Josy Anteveli} and Silva-Filho, {Edson Cavalcanti} and Rizzo, {M{\'a}rcia dos Santos} and Ribeiro, {Alessandra Braga} and Costa, {Marcilia Pinheiro da}",

year = "2022",

month = jun,

day = "17",

doi = "10.3390/molecules27123895",

language = "English",

volume = "27",

journal = "Molecules",

issn = "1420-3049",

publisher = "Multidisciplinary Digital Publishing Institute",

number = "12",

}

Lucena, MDA, Ramos, IFDS, Geronço, MS, Araújo, RD, Filho, FLDS, Silva, LMLRD, Sousa, RWRD, Ferreira, PMP, Osajima, JA, Silva-Filho, EC, Rizzo, MDS, Ribeiro, AB & Costa, MPD 2022, 'Biopolymer from water kefir as a potential clean-label ingredient for health applications: evaluation of new properties', Molecules, vol. 27, n.º 12, 3895. https://doi.org/10.3390/molecules27123895

TY - JOUR

T1 - Biopolymer from water kefir as a potential clean-label ingredient for health applications

T2 - evaluation of new properties

AU - Lucena, Monalisa de Alencar

AU - Ramos, Igor Frederico da Silveira

AU - Geronço, Maurycyo Silva

AU - Araújo, Ricardo de

AU - Filho, Francisco Lopes da Silva

AU - Silva, Luís Manuel Lopes Rodrigues da

AU - Sousa, Rayran Walter Ramos de

AU - Ferreira, Paulo Michel Pinheiro

AU - Osajima, Josy Anteveli

AU - Silva-Filho, Edson Cavalcanti

AU - Rizzo, Márcia dos Santos

AU - Ribeiro, Alessandra Braga

AU - Costa, Marcilia Pinheiro da

PY - 2022/6/17

Y1 - 2022/6/17

N2 - The present work aimed to characterize the exopolysaccharide obtained from water kefir grains (EPSwk), a symbiotic association of probiotic microorganisms. New findings of the technological, mechanical, and biological properties of the sample were studied. The EPSwk polymer presented an Mw of 6.35 × 105 Da. The biopolymer also showed microcrystalline structure and characteristic thermal stability with maximum thermal degradation at 250 °C. The analysis of the monosaccharides of the EPSwk by gas chromatography demonstrated that the material is composed of glucose units (98 mol%). Additionally, EPSwk exhibited excellent emulsifying properties, film-forming ability, a low photodegradation rate (3.8%), and good mucoadhesive properties (adhesion Fmax of 1.065 N). EPSwk presented cytocompatibility and antibacterial activity against Escherichia coli and Staphylococcus aureus. The results of this study expand the potential application of the exopolysaccharide from water kefir as a potential clean-label raw material for pharmaceutical, biomedical, and cosmetic applications.

AB - The present work aimed to characterize the exopolysaccharide obtained from water kefir grains (EPSwk), a symbiotic association of probiotic microorganisms. New findings of the technological, mechanical, and biological properties of the sample were studied. The EPSwk polymer presented an Mw of 6.35 × 105 Da. The biopolymer also showed microcrystalline structure and characteristic thermal stability with maximum thermal degradation at 250 °C. The analysis of the monosaccharides of the EPSwk by gas chromatography demonstrated that the material is composed of glucose units (98 mol%). Additionally, EPSwk exhibited excellent emulsifying properties, film-forming ability, a low photodegradation rate (3.8%), and good mucoadhesive properties (adhesion Fmax of 1.065 N). EPSwk presented cytocompatibility and antibacterial activity against Escherichia coli and Staphylococcus aureus. The results of this study expand the potential application of the exopolysaccharide from water kefir as a potential clean-label raw material for pharmaceutical, biomedical, and cosmetic applications.

KW - Antimicrobial activity

KW - Biopolymer

KW - Dextran

KW - Mucoadhesiveness

KW - Photostability

KW - Polysaccharide

UR - http://www.scopus.com/inward/record.url?scp=85132933767&partnerID=8YFLogxK

U2 - 10.3390/molecules27123895

DO - 10.3390/molecules27123895

M3 - Article

C2 - 35745016

VL - 27

JO - Molecules

JF - Molecules

SN - 1420-3049

IS - 12

M1 - 3895

ER -

Biopolymer from water kefir as a potential clean-label ingredient for health applications: evaluation of new properties

Resumo

Acesso ao documento

Outros arquivos e links

Impressão digital

Projetos

CBQF Multianual Funding: Centro de Biotecnologia e Química Fina

Citação