BSA-based sample clean-up columns for ochratoxin A determination in wine: method development and validation

Tânia Leal, Luís Abrunhosa, Lucília Domingues, Armando Venâncio, C. Oliveira*

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

16 Citações (Scopus)

Resumo

Analytical chromatographic techniques for mycotoxins control are well established, but they often depend on costly immunoaffinity sample clean-up. Serum albumins, particularly that from bovine origin (BSA), have stable binding affinity towards some mycotoxins, and can be cheaper alternative receptors for sample clean-up due to their wide availability. Thus, this work used BSA immobilized in agarose beads as a novel solid-phase extraction method for quantification of ochratoxin A (OTA) in wine. Constructed BSA-agarose columns could extract OTA efficiently from red wine after its dilution (4-fold) in 0.1 M Tris pH 8.0. The method was linear (R2 = 0.9999) in the OTA concentration range studied (0.05 to 3.0 μg L−1), with recovery rates above 98%. It also showed low detection (0.017 μg L−1) and quantification (0.051 μg L−1) limits. The efficacy of the BSA-based method was further validated by direct comparison with commercial immunoaffinity columns. Portuguese wines analyzed by both methods had agreeing results.
Idioma originalEnglish
Número do artigo125204
RevistaFood Chemistry
Volume300
DOIs
Estado da publicaçãoPublicado - 1 dez. 2019
Publicado externamenteSim

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