TY - JOUR
T1 - Carbon nanotube field effect transistor biosensor for the detection of toxins in seawater
AU - Marques, Inês
AU - Costa, João Pinto da
AU - Justino, Celine
AU - Santos, Patrícia
AU - Duarte, Kátia
AU - Freitas, Ana
AU - Cardoso, Susana
AU - Duarte, Armando
AU - Rocha-Santos, Teresa
N1 - Funding Information:
This work was funded by Portuguese Science Foundation (FCT) through scholarships [ref. SFRH/BPD/95961/2013 (Celine I.L. Justino), SFRH/BPD/102452/2014 (Patrícia S.M. Santos) and SFRH/BD/84524/2012 (Kátia R. Duarte)] under POCH funds, co-financed by the European Social Fund and Portuguese National Funds from MEC. This work was also supported by national funds through FCT/MEC (PIDDAC) under project IF/00407/2013/CP1162/CT0023. Thanks are also due, for the financial support to CESAM (UID/AMB/50017), to FCT/MEC through national funds, and the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020. INESC-MN acknowledges FCT funding through the Associated Lab - Instituto de Nanotecnologias (IN).
Publisher Copyright:
© 2017 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2017/5/28
Y1 - 2017/5/28
N2 - Disposable field effect transistors (FET) biosensors (bio-FET) based on carbon nanotubes were fabricated for detection of domoic acid (DA), which belongs to the group of biotoxins associated with the amnesic shellfish poisoning. The analytical results obtained with the bio-FET were compared with those obtained with a traditional methodology (enzyme-linked immunosorbent assay) in order to validate the bio-FET for DA detection. Standard solutions of DA with concentrations between 10 and 500 ng L−1 were tested in order to construct the calibration curve, where five bio-FET were used for reproducibility estimation and two analytical measurements were performed for each bio-FET for repeatability estimation. Ten spiked artificial seawater samples were used to validate the bio-FET. The obtained reproducibility (0.52–1.43%), repeatability (0.57–1.27%), limit of detection (10 ng L−1) and recovery range (92.3–100.3%) reveal an adequate analytical performance of the bio-FET for the detection of DA in environmental samples such as seawater samples.
AB - Disposable field effect transistors (FET) biosensors (bio-FET) based on carbon nanotubes were fabricated for detection of domoic acid (DA), which belongs to the group of biotoxins associated with the amnesic shellfish poisoning. The analytical results obtained with the bio-FET were compared with those obtained with a traditional methodology (enzyme-linked immunosorbent assay) in order to validate the bio-FET for DA detection. Standard solutions of DA with concentrations between 10 and 500 ng L−1 were tested in order to construct the calibration curve, where five bio-FET were used for reproducibility estimation and two analytical measurements were performed for each bio-FET for repeatability estimation. Ten spiked artificial seawater samples were used to validate the bio-FET. The obtained reproducibility (0.52–1.43%), repeatability (0.57–1.27%), limit of detection (10 ng L−1) and recovery range (92.3–100.3%) reveal an adequate analytical performance of the bio-FET for the detection of DA in environmental samples such as seawater samples.
KW - Biosensor
KW - Biotoxin
KW - Domoic acid
KW - Field effect transistor
KW - Seawater
KW - Single-walled carbon nanotubes
UR - https://www.scopus.com/pages/publications/85019759859
U2 - 10.1080/03067319.2017.1334056
DO - 10.1080/03067319.2017.1334056
M3 - Article
AN - SCOPUS:85019759859
SN - 0306-7319
VL - 97
SP - 597
EP - 605
JO - International Journal of Environmental Analytical Chemistry
JF - International Journal of Environmental Analytical Chemistry
IS - 7
ER -