TY - JOUR
T1 - Characterization of clinical and food Listeria monocytogenes isolates with different antibiotic resistance patterns through simulated gastrointestinal tract conditions and environmental stresses
AU - Cunha, S.
AU - Komora, N.
AU - Magalhães, R.
AU - Almeida, G.
AU - Ferreira, V.
AU - Teixeira, P.
N1 - Funding Information:
This work was supported by funding from the National Funds from the Fundação para a Ciência e a Tecnologia (FCT) through project Pest-OE/EQB/LA0016/2013 and “KLEAR - Knowledge and tools for safety, quality and competitiveness in the food chain.” nº NORTE-07-0124-FEDER-000052, co-funded by Fundo Europeu de Desenvolvimento Regional (FEDER), under Programa ON2, Eixo Prioritário 01-01 – Competitividade, Inovação e Conhecimento, Tipologia 01-01-01-24 – Apoio a Entidades do Sistema Científico e Tecnológico. Financial support for authors Rui Magalhães and Vânia Ferreira was provided by FCT through doctoral fellowship SFRH/BD/71704/2010 and postdoctoral fellowship SFRH/BPD/72617/2010, respectively. Editing of this paper by Dr. P. A. Gibbs is gratefully acknowledged.
Publisher Copyright:
© 2015.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Thirty-three Listeria monocytogenes isolates previously collected from two sources, food (n = 18) and human patients suffering from listeriosis (n = 15), with variable antibiotic susceptibility profiles (sensitive/resistant) to erythromycin, ciprofloxacin and nitrofurantoin, were studied for their ability to survive (i) the environmental stress provided by sequential conditions that simulate the digestive tract, and (ii) extreme pH values (1.5-12). The results showed a response that was only strain dependent. There were no variability in survival results based on type of stress (low or high pH), source (food or clinical), or sensitivity/resistance to antibiotics (p > 0.01). Some strains of L. monocytogenes are able to survive extreme acid and alkaline conditions, and conditions that mimic the sequential stressors found in the gastro-intestinal tract. The resistance to the antibiotics tested in this study by some L. monocytogenes strains did not confer any cross-protection to acid or alkaline stressors.
AB - Thirty-three Listeria monocytogenes isolates previously collected from two sources, food (n = 18) and human patients suffering from listeriosis (n = 15), with variable antibiotic susceptibility profiles (sensitive/resistant) to erythromycin, ciprofloxacin and nitrofurantoin, were studied for their ability to survive (i) the environmental stress provided by sequential conditions that simulate the digestive tract, and (ii) extreme pH values (1.5-12). The results showed a response that was only strain dependent. There were no variability in survival results based on type of stress (low or high pH), source (food or clinical), or sensitivity/resistance to antibiotics (p > 0.01). Some strains of L. monocytogenes are able to survive extreme acid and alkaline conditions, and conditions that mimic the sequential stressors found in the gastro-intestinal tract. The resistance to the antibiotics tested in this study by some L. monocytogenes strains did not confer any cross-protection to acid or alkaline stressors.
KW - Acid stress
KW - Alkali stress
KW - Antibiotic resistance
KW - Gastro-intestinal tract
KW - Listeria monocytogenes
UR - http://www.scopus.com/inward/record.url?scp=84953295776&partnerID=8YFLogxK
U2 - 10.1016/j.mran.2015.08.001
DO - 10.1016/j.mran.2015.08.001
M3 - Article
AN - SCOPUS:84953295776
SN - 2352-3530
VL - 1
SP - 40
EP - 46
JO - Microbial Risk Analysis
JF - Microbial Risk Analysis
ER -