TY - JOUR
T1 - Chitosan produced from Mucorales fungi using agroindustrial by-products and its efficacy to inhibit Colletotrichum species
AU - Berger, Lúcia Raquel Ramos
AU - Stamford, Thayza Christina Montenegro
AU - Oliveira, Kataryne Árabe Rimá de
AU - Pessoa, Adjane de Miranda Pereira
AU - Lima, Marcos Antonio Barbosa de
AU - Pintado, Maria Manuela Estevez
AU - Câmara, Marcos Paz Saraiva
AU - Franco, Luciana de Oliveira
AU - Magnani, Marciane
AU - Souza, Evandro Leite de
N1 - Funding Information:
The authors thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq − Brazil) for financial support and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES − Brazil) for the postdoctoral scholarship awarded to L.R.R. Berger. This work was also supported by Fundação para a Ciência e Tecnologia (FCT − Portugal) through project UID/Multi/50016/2013. Authors are grateful to Corn Products do Brasil Ltda. (Cabo de Santo Agostinho-PE, Brazil), which kindly provided CSL. Dr. A. Leyva (USA) helped with English editing of the manuscript.
Funding Information:
The authors thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq − Brazil) for financial support and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ( CAPES − Brazil) for the postdoctoral scholarship awarded to L.R.R. Berger. This work was also supported by Fundação para a Ciência e Tecnologia (FCT − Portugal) through project UID/Multi/50016/2013. Authors are grateful to Corn Products do Brasil Ltda . (Cabo de Santo Agostinho-PE, Brazil), which kindly provided CSL. Dr. A. Leyva (USA) helped with English editing of the manuscript.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2018/3
Y1 - 2018/3
N2 - This study evaluated corn steep liquor (CSL) and papaya peel juice (PPJ) in mixture as substrates for the cultivation (96 h, 28 °C, pH 5.6, 150 rpm) of Mucorales fungi for chitosan production, and determined the growth-inhibitory effect of the fungal chitosan (FuCS) obtained under optimized conditions against phytopathogenic Colletotrichum species. All Mucorales fungi tested were capable of growing in CSL-PPJ medium, showing FuCS production in the range of 5.02 (Fennelomyces heterothalicus SIS 28) − 15.63 mg/g (Cunninghamella elegans SIS 41). Highest FuCS production (37.25 mg/g) was achieved when C. elegans was cultivated in medium containing 9.43% CSL and 42.5% PPJ. FuCS obtained under these conditions showed a deacetylation degree of 86%, viscosity of 120 cP and molecular weight of 4.08 × 104 g/mol. FuCS at 5000, 7500 and 10,000 ppm inhibited the growth of all Colletotrichum species tested. FuCS also induced alterations in the morphology of C. fructicola hyphae. CSL-PPJ mixtures are suitable substrates for the cultivation of Mucorales fungi for FuCS production. Chitosan from C. elegans cultivated in CSL-PPJ medium is effective in inhibiting phytopathogenic Colletotrichum species.
AB - This study evaluated corn steep liquor (CSL) and papaya peel juice (PPJ) in mixture as substrates for the cultivation (96 h, 28 °C, pH 5.6, 150 rpm) of Mucorales fungi for chitosan production, and determined the growth-inhibitory effect of the fungal chitosan (FuCS) obtained under optimized conditions against phytopathogenic Colletotrichum species. All Mucorales fungi tested were capable of growing in CSL-PPJ medium, showing FuCS production in the range of 5.02 (Fennelomyces heterothalicus SIS 28) − 15.63 mg/g (Cunninghamella elegans SIS 41). Highest FuCS production (37.25 mg/g) was achieved when C. elegans was cultivated in medium containing 9.43% CSL and 42.5% PPJ. FuCS obtained under these conditions showed a deacetylation degree of 86%, viscosity of 120 cP and molecular weight of 4.08 × 104 g/mol. FuCS at 5000, 7500 and 10,000 ppm inhibited the growth of all Colletotrichum species tested. FuCS also induced alterations in the morphology of C. fructicola hyphae. CSL-PPJ mixtures are suitable substrates for the cultivation of Mucorales fungi for FuCS production. Chitosan from C. elegans cultivated in CSL-PPJ medium is effective in inhibiting phytopathogenic Colletotrichum species.
KW - Agroindustrial by-products
KW - Antifungal effects
KW - Fungal chitosan
UR - https://www.scopus.com/pages/publications/85037858492
U2 - 10.1016/j.ijbiomac.2017.11.178
DO - 10.1016/j.ijbiomac.2017.11.178
M3 - Article
C2 - 29199126
AN - SCOPUS:85037858492
SN - 0141-8130
VL - 108
SP - 635
EP - 641
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -