Continuous-flow precipitation as a route to prepare highly controlled nanohydroxyapatite: in vitro mineralization and biological evaluation

Filipa Castro, Viviana P. Ribeiro, António Ferreira, Ana L. Oliveira, Rui L. Reis, José A. Teixeira, Fernando Rocha

Resultado de pesquisarevisão de pares

8 Citações (Scopus)
3 Transferências (Pure)

Resumo

This work reports the biological evaluation of nanosized hydroxyapatite (HAp) previously synthesized by continuous-flow precipitation in a scaled-up meso oscillatory flow reactor (meso-OFR). Physicochemical characterization of the synthesized HApsuggests high surface reactivity namely because of its high specific surface area and low crystallinity. On the other hand, in vitro biomineralization assays demonstrated the apatite-forming activity of the prepared HAp and their higher surface reactivity when compared to a commercial HAp. Furthermore, human osteoblastic-like (Saos-2) cells culture evidenced that the synthesized HAp stimulated cell proliferation, especially when applied at lower concentrations (30 and 50 μgml-1), although its cellular uptake behavior. Therefore, the prepared HApshows immense potential as biomedical material, as well as drug and gene delivery vehicle. The results are also very promising regarding further scaling up of the process, as the designed methodology allow for the preparation in a continuous mode of nanosized HAp with controlled physico-chemical properties.
Idioma originalEnglish
Número do artigo075404
Páginas (de-até)1-13
Número de páginas13
RevistaMaterials Research Express
Volume3
Número de emissão7
DOIs
Estado da publicaçãoPublicado - jul. 2016

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