Cytotoxicity and genotoxicity of chitooligosaccharides upon lymphocytes

João C. Fernandes*, Margarida Borges, Henrique Nascimento, Elsa Bronze-da-Rocha, Oscar S. Ramos, Manuela E. Pintado, F. Xavier Malcata, Alice Santos-Silva

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

25 Citações (Scopus)
6 Transferências (Pure)

Resumo

Two COS mixtures and a low molecular weight chitosan (LMWC) were tested for potential cytotoxicity and genotoxicity upon human lymphocytes. Genotoxicity was evaluated in vitro by cytokinesis-blocked micronucleus and alkaline comet assays, while cytotoxicity was assessed by flow cytometry analysis. Our results suggest that COS do not exhibit any genotoxicity upon human lymphocytes, independently of MW or concentration. However, above 0.07. mg/mL COS induced strong cytotoxic effects. According to the concentration used, such cytotoxicity will induce cell death, essentially by necrosis (>0.10. mg/mL) and/or apoptosis (<0.10. mg/mL). The level of necrosis/apoptosis induced by high COS concentrations, suggests a promising use as apoptosis inducers in specific cancer situations.
Idioma originalEnglish
Páginas (de-até)433-438
Número de páginas6
RevistaInternational Journal of Biological Macromolecules
Volume49
Número de emissão3
DOIs
Estado da publicaçãoPublicado - 1 out. 2011

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