TY - JOUR
T1 - Development of composite scaffolds based on cerium doped-hydroxyapatite and natural gums-biological and mechanical properties
AU - Santos, Marcus Vinicius Beserra dos
AU - Rocha, Lorenna Bastos Nogueira
AU - Vieira, Ewerton Gomes
AU - Oliveira, Ana Leite
AU - Lobo, Anderson Oliveira
AU - de Carvalho, Maria Acelina Martins
AU - Osajima, Josy Anteveli
AU - Silva-Filho, Edson Cavalcanti
N1 - Funding Information:
This research was funded by CNPq [Conselho Nacional de Desenvolvimento Científico e Tecnológico] grant number [307460/2016-9]. The authors thank CAPES, CNPq, FAPEPI, UFPI, NUPCelt, UCP-Porto, and KELCOGEL® for financial and/or structural support.
Funding Information:
Funding: This research was funded by CNPq [Conselho Nacional de Desenvolvimento Científico e Tecnológico] grant number [307460/2016-9].
Funding Information:
Acknowledgments: The authors thank CAPES, CNPq, FAPEPI, UFPI, NUPCelt, UCP–Porto, and KELCOGEL® for financial and/or structural support.
Publisher Copyright:
© 2019 by the authors.
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Hydroxyapatite (HAp) is a ceramic material composing the inorganic portion of bones. Ionic substitutions enhance characteristics of HAp, for example, calcium ions (Ca2+) by cerium ions (Ce3+). The use of HAp is potentialized through biopolymers, cashew gum (CG), and gellan gum (GG), since CG/GG is structuring agents in the modeling of structured biocomposites, scaffolds. Ce-HApCG biocomposite was synthesized using a chemical precipitation method. The obtained material was frozen (-20 °C for 24 h), and then vacuum dried for 24 h. The Ce-HApCG was characterized by X-Ray diffractograms (XRD), X-ray photoemission spectra (XPS), Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), and energy dispersive spectroscopy (EDS). XRD and FTIR showed that Ce-HApCG was successfully synthesized. XRD showed characteristic peaks at 2θ = 25.87 and 32.05, corresponding to the crystalline planes (0 0 2) and (2 1 1), respectively, while phosphate bands were present at 1050 cm-1 and 1098 cm-1, indicating the success of composite synthesis. FESEM showed pores and incorporated nanostructured granules of Ce-HApCG. The mechanical test identified that Ce-HApCG has a compressive strength similar to the cancellous bone's strength and some allografts used in surgical procedures. In vitro tests (MTT assay and hemolysis) showed that scaffold was non-toxic and exhibited low hemolytic activity. Thus, the Ce-HApCG has potential for application in bone tissue engineering.
AB - Hydroxyapatite (HAp) is a ceramic material composing the inorganic portion of bones. Ionic substitutions enhance characteristics of HAp, for example, calcium ions (Ca2+) by cerium ions (Ce3+). The use of HAp is potentialized through biopolymers, cashew gum (CG), and gellan gum (GG), since CG/GG is structuring agents in the modeling of structured biocomposites, scaffolds. Ce-HApCG biocomposite was synthesized using a chemical precipitation method. The obtained material was frozen (-20 °C for 24 h), and then vacuum dried for 24 h. The Ce-HApCG was characterized by X-Ray diffractograms (XRD), X-ray photoemission spectra (XPS), Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), and energy dispersive spectroscopy (EDS). XRD and FTIR showed that Ce-HApCG was successfully synthesized. XRD showed characteristic peaks at 2θ = 25.87 and 32.05, corresponding to the crystalline planes (0 0 2) and (2 1 1), respectively, while phosphate bands were present at 1050 cm-1 and 1098 cm-1, indicating the success of composite synthesis. FESEM showed pores and incorporated nanostructured granules of Ce-HApCG. The mechanical test identified that Ce-HApCG has a compressive strength similar to the cancellous bone's strength and some allografts used in surgical procedures. In vitro tests (MTT assay and hemolysis) showed that scaffold was non-toxic and exhibited low hemolytic activity. Thus, the Ce-HApCG has potential for application in bone tissue engineering.
KW - Calcium phosphate
KW - Doping
KW - Scaffold
UR - https://www.scopus.com/pages/publications/85070408849
U2 - 10.3390/ma12152389
DO - 10.3390/ma12152389
M3 - Article
C2 - 31357470
AN - SCOPUS:85070408849
SN - 1996-1944
VL - 12
JO - Materials
JF - Materials
IS - 15
M1 - 2389
ER -