Discrimination of the Acinetobacter calcoaceticus-Acinetobacter baumannii complex species by Fourier transform infrared spectroscopy

C. Sousa, L. Silva, F. Grosso, A. Nemec, J. Lopes, L. Peixe*

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

17 Citações (Scopus)

Resumo

The main goal of this work was to assess the ability of Fourier transform infrared spectroscopy with attenuated total reflectance (FTIR-ATR) to discriminate between the species of the Acinetobacter calcoaceticus- Acinetobacter baumannii (Acb) complex, i.e. A. baumannii, A. nosocomialis, A. pittii, A. calcoaceticus, genomic species "Between 1 and 3" and genomic species "Close to 13TU". A total of 122 clinical isolates of the Acb complex previously identified by rpoB sequencing were studied. FTIR-ATR spectra was analysed by partial least squares discriminant analysis (PLSDA) and the model scores were presented in a dendrogram form. This spectroscopic technique proved to be effective in the discrimination of the Acb complex species, with sensitivities from 90 to 100 %. Moreover, a flowchart aiming to help with species identification was developed and tested with 100 % correct predictions for A. baumannii, A. nosocomialis and A. pittii test isolates. This rapid, low cost and environmentally friendly technique proved to be a reliable alternative for the identification of these closely related Acinetobacter species that share many clinical and epidemiological characteristics and are often difficult to distinguish. Its validation towards application on a routine basis could revolutionise high-throughput bacterial identification.
Idioma originalEnglish
Páginas (de-até)1345-1353
Número de páginas9
RevistaEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume33
Número de emissão8
DOIs
Estado da publicaçãoPublicado - ago. 2014
Publicado externamenteSim

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