Enhanced biocatalytic sustainability of laccase by immobilization on functionalized carbon nanotubes/polysulfone membranes

João B. Costa, Maria J. Lima, Maria J. Sampaio, Márcia C. Neves, Joaquim L. Faria, Sergio Morales-Torres*, Ana P. M. Tavares*, Cláudia G. Silva*

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

133 Citações (Scopus)

Resumo

Chemically functionalized multi-walled carbon nanotubes (CNTs) are used as carriers for laccase immobilization. In this work, CNTs were modified using different approaches with a combination of methods involving hydrothermal oxidation with nitric acid, treatment with 3-aminopropyltriethoxysilane, glutaraldehyde, N-ethyl-N-(3-(dimethylamino)-propyl) carbodiimide hydrochloride and N-hydroxysuccinimide. The enzyme immobilization efficiency and recovered activity were evaluated towards 2,2′-azino-bis(3-ethylbenzathiazoline-6-sulfonic acid) biocatalytic oxidation. The best compromise between immobilization efficiency and recovered activity was obtained using the CNTs functionalized with 0.3 M HNO 3, treated with N-ethyl-N-(3-(dimethylamino)propyl) carbodiimide hydrochloride and N-hydroxysuccinimide. This catalyst also showed the best thermal stability (at 50 and 60 °C). The bioconjugate based on this material was characterized by vibrational spectroscopies (FTIR and Raman) and by N 2 adsorption. The results from reutilization tests showed that laccase activity was kept above 65% of its initial value after five consecutive cycles of reuse. The biocatalytic performance of the immobilized enzyme was evaluated for the degradation of a mixture of phenolic compounds in water containing phenol, resorcinol, 4-methoxyphenol and 4-chlorophenol. As means of cost efficient to enzyme reutilization, laccase was immobilized over polysulfone membranes blended with the functionalized CNTs and studied in the degradation of 4-methoxyphenol.
Idioma originalEnglish
Páginas (de-até)974-985
Número de páginas12
RevistaChemical Engineering Journal
Volume355
DOIs
Estado da publicaçãoPublicado - 1 jan. 2019
Publicado externamenteSim

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