Escherichia coli and Salmonella Enteritidis dual-species biofilms: interspecies interactions and antibiofilm efficacy of phages

Catarina Milho, Maria Daniela Silva, Diana Alves, Hugo Oliveira, Clara Sousa, Lorenzo M. Pastrana, Joana Azeredo, Sanna Sillankorva*

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

35 Citações (Scopus)

Resumo

Escherichia coli and Salmonella Enteritidis are foodborne pathogens forming challenging biofilms that contribute to their virulence, antimicrobial resistance, and survival on surfaces. Interspecies interactions occur between species in mixed biofilms promoting different outcomes to each species. Here we describe the interactions between E. coli and S. Enteritidis strains, and their control using specific phages. Single-species biofilms presented more cells compared to dual-species biofilms. The spatial organization of strains, observed by confocal microscopy, revealed similar arrangements in both single- and dual-species biofilms. The EPS matrix composition, assessed by Fourier-transform infrared spectroscopy, disclosed that the spectra extracted from the different dual-species biofilms can either be a combination of both species EPS matrix components or that the EPS matrix of one species predominates. Phages damaged more the single-species biofilms than the mixed biofilms, showing also that the killing efficacy was greatly dependent on the phage growth characteristics, bacterial growth parameters, and bacterial spatial distribution in biofilms. This combination of methodologies provides new knowledge of species-species and phage-host interactions in biofilms of these two major foodborne pathogens.
Idioma originalEnglish
Número do artigo18183
Páginas (de-até)1-15
Número de páginas15
RevistaScientific Reports
Volume9
Número de emissão1
DOIs
Estado da publicaçãoPublicado - 1 dez. 2019
Publicado externamenteSim

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