Exploiting the bead injection LOV approach to carry out spectrophotometric assays in wine: application to the determination of iron

Susana S. M. P. Vidigal, Ildikó V. Tóth, António O. S. S. Rangel*

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

22 Citações (Scopus)
5 Transferências (Pure)

Resumo

A sequential injection lab-on-valve (SI-LOV) system was used to develop a new methodology for the determination of iron in wine samples exploiting the bead injection (BI) concept for solid phase extraction and spectrophotometric measurement. Nitrilotriacetic Acid (NTA) Superflow resin was used to build the bead column of the flow through sensor. The iron (III) ions were retained by the bead column and react with SCN- producing an intense red colour. The change in absorbance was monitored spectrophotometrically on the optosensor at 480 nm. It was possible to achieve a linear range of 0.09-5.0 mg L-1 of iron, with low sample and reagent consumption; 500 μL of sample, 15 μmol of SCN-, and 9 μmol of H2O2, per assay. The proposed method was successfully applied to the determination of iron in wine, with no previous treatment other than dilution, and to other food samples.
Idioma originalEnglish
Páginas (de-até)1298-1303
Número de páginas6
RevistaTalanta
Volume84
Número de emissão5
DOIs
Estado da publicaçãoPublicado - 15 jun. 2011
Evento16th International Conference on Flow Injection Analysis - Garden Sea View Resort Hotel, Pattaya
Duração: 25 abr. 201030 abr. 2010
Número de conferência: 16

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