Exploring silk Sericin for diabetic wounds: an in situ-forming hydrogel to protect against oxidative stress and improve tissue healing and regeneration

Sara Baptista-Silva, Beatriz G. Bernardes, Sandra Borges, Ilda Rodrigues, Rui Fernandes, Susana Gomes-Guerreiro, Marta Teixeira Pinto, Manuela Pintado, Raquel Soares, Raquel Costa*, Ana Leite Oliveira*

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

1 Citação (Scopus)

Resumo

Chronic wounds are one of the most frequent complications that are associated with diabetes mellitus. The overproduction of reactive oxygen species (ROS) is a key factor in the delayed healing of a chronic wound. In the present work, we develop a novel in situ-forming silk sericin-based hydrogel (SSH) that is produced by a simple methodology using horseradish peroxidase (HRP) crosslinking as an advanced dressing for wound healing. The antioxidant and angiogenic effects were assessed in vitro and in vivo after in situ application using an excisional wound-healing model in a genetically-induced diabetic db/db mice and though the chick embryo choriollantoic membrane (CAM) assay, respectively. Wounds in diabetic db/db mice that were treated with SSH closed with reduced granulation tissue, decreased wound edge distance, and wound thickness, when compared to Tegaderm, a dressing that is commonly used in the clinic. The hydrogel also promoted a deposition of collagen fibers with smaller diameter which may have had a boost effect in re-epithelialization. SSH treatment slightly induced two important endogenous antioxidant defenses, superoxide dismutase and catalase. A CAM assay made it possible to observe that SSH led to an increase in the number of newly formed vessels without inducing an inflammatory reaction. The present hydrogel may result in a multi-purpose technology with angiogenic, antioxidant, and anti-inflammatory properties, while advancing efficient and organized tissue regeneration.

Idioma originalEnglish
Número do artigo801
Número de páginas30
RevistaBiomolecules
Volume12
Número de emissão6
DOIs
Estado da publicaçãoPublished - 8 jun 2022

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