First bioactive characterization of the skin mucus from Portugal coastal fish halobatrachus didactylus

Marta Cunha*, Ezequiel R. Coscueta, Maria Emilia Brassesco, Frederico Almada, David Gonçalves, Manuela Pintado

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

14 Transferências (Pure)


The marine environment has several promising features for the discovery of new molecules. The oceans have half of the planet’s biodiversity and are a harsh environment for aquatic organisms challenging them to have a set of bioactive molecules to survive [1]. It is well known the mucus secreted by fish epidermis works as a defense barrier against harmful elements from the external environment [2]. Therefore, this study aimed to explore some bioactive properties such as antimicrobial activity (agar drop diffusion method), antioxidant activity (ABTS and ORAC scavenging assays) and ACE inhibitory activity (iACE) exerted by the mucus collected scraping the Halobatrachus didactylus skin, from the Tagus estuary. Direct drop application did not show inhibitory activity on the growth of E. coli and S. aureus. The protein content
in the mucus, determined by the bicinchoninic acid methodology, was 13260 ± 342 μg BSA/mL. The antioxidant activity resulted in 1.49 ± 0.04 μmol TE/mL for ABTS and 5.47 ± 0.8 μmol TE/mL for ORAC. iACE resulted in an IC50 of 60 ± 7 μg protein/mL. Also, a peptidic profile of the mucus was obtained through size exclusion chromatography showing a shortchain peptide profile (> 3000 Da) as general distribution, which is consistent with the observed bioactivities. In conclusion, fish mucus peptide fraction showed potential as an antioxidant and even more so as an antihypertensive, but not as an antimicrobial. Furthermore, more studies are needed to discover the key molecules behind these bioactivities.
Idioma originalEnglish
Número de páginas1
Estado da publicaçãoPublicado - abr. 2022
Evento3.º BioIberoAmerica 2022 - Altice Forum Braga, Braga
Duração: 7 abr. 20229 abr. 2022
Número de conferência: 3


Conferência3.º BioIberoAmerica 2022
Título abreviadoBioIberoAmerica 2022
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