Genetic gains and losses in oral squamous cell carcinoma: impact on clinical management

Ilda Patrícia Ribeiro*, Francisco Marques, Francisco Caramelo, João M. S. Pereira, Miguel Patrício, Hugo Prazeres, José Ferrão, Maria José Julião, Miguel Castelo-Branco, Joana Barbosa de Melo, Isabel Poiares Baptista, Isabel Marques Carreira

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

42 Citações (Scopus)

Resumo

Purpose: The identification of genetic markers associated with oral cancer is considered essential to improve the diagnosis, prognosis, early tumor and relapse detection and, ultimately, to delineate individualized therapeutic approaches. Here, we aimed at identifying such markers. Methods: Multiplex Ligation-dependent Probe Amplification (MLPA) analyses encompassing 133 cancer-related genes were performed on a panel of primary oral tumor samples and its corresponding resection margins (macroscopically tumor-free tissue) allowing, in both types of tissue, the detection of a wide arrange of copy number imbalances on various human chromosomes. Results: We found that in tumor tissue, from the 133 cancer-related genes included in this study, those that most frequently exhibited copy number gains were located on chromosomal arms 3q, 6p, 8q, 11q, 16p, 16q, 17p, 17q and 19q, whereas those most frequently exhibiting copy number losses were located on chromosomal arms 2q, 3p, 4q, 5q, 8p, 9p, 11q and 18q. Several imbalances were highlighted, i.e., losses of ERBB4, CTNNB1, NFKB1, IL2, IL12B, TUSC3, CDKN2A, CASP1, and gains of MME, BCL6, VEGF, PTK2, PTP4A3, RNF139, CCND1, FGF3, CTTN, MVP, CDH1, BRCA1, CDKN2D, BAX, as well as exon 4 of TP53. Comparisons between tumor and matched macroscopically tumor-free tissues allowed us to build a logistic regression model to predict the tissue type (benign versus malignant). In this model, the TUSC3 gene showed statistical significance, indicating that loss of this gene may serve as a good indicator of malignancy. Conclusions: Our results point towards relevance of the above mentioned cancer-related genes as putative genetic markers for oral cancer. For practical clinical purposes, these genetic markers should be validated in additional studies.
Idioma originalEnglish
Páginas (de-até)29-39
Número de páginas11
RevistaCellular oncology (Dordrecht)
Volume37
Número de emissão1
DOIs
Estado da publicaçãoPublicado - fev. 2014
Publicado externamenteSim

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