Hydrolysis of butteroil by immobilized lipase using a hollow‐fiber reactor: part III. Multiresponse kinetic studies

F. Xavier Malcata, Charles G. Hill*, Clyde H. Amundson

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

36 Citações (Scopus)

Resumo

A lipase from Aspergillus niger immobilized by adsorption on microporous, polypropylene hollow fibers was used to effect the continuous hydrolysis of the glycerides of butter oil at 40°C and pH 7.0. The effluent concentrations of 10 different free fatty acid products were measured by highperformancee liquid chromatography (HPLC). Multiresponse nonlinear regression methods were used to fit the data to a multisubstrate rate expression derived from a Ping Pong Bi Bi mechanism in which the rate‐controlling step is deacylation of the lipase. Thermal deactivation of the enzyme was also included in the mathematical model of reactor performance. A postulated normal distribution of vmax with respect to the chain length of the fatty acid (with an additive correction for the degree of unsaturation) was tested for statistical significance. The model is useful for predicting the free fatty acid profile of the lipolyzed butteroil product over a wide range of flow rates.
Idioma originalEnglish
Páginas (de-até)1002-1012
Número de páginas11
RevistaBiotechnology and Bioengineering
Volume39
Número de emissão10
DOIs
Estado da publicaçãoPublicado - 25 abr 1992

Impressão digital

Mergulhe nos tópicos de investigação de “Hydrolysis of butteroil by immobilized lipase using a hollow‐fiber reactor: part III. Multiresponse kinetic studies“. Em conjunto formam uma impressão digital única.

Citação