Hydrolysis of caseins by extracts of Cynara cardunculus precipitated by ammonium sulfate

S. V. Silva, R. M. Barros, F. X. Malcata*

*Autor correspondente para este trabalho

Resultado de pesquisarevisão de pares

16 Citações (Scopus)

Resumo

Polyacrylamide gel electrophoresis (in the presence of urea) and gel permeation chromatography were employed to assess the profile of hydrolysis of caseins and the activity of enzymes contributed by the flowers of the plant Cynara cardunculus on bovine caseins, after previous precipitation with ammonium sulfate or in a plain crude aqueous extract. Results indicated that the qualitative degradation profile of bovine caseins by plant enzymes (cardosins) remains essentially unchanged upon extraction, and quantitative analysis showed that the precipitated fractions had a higher coagulant-to-proteolytic activity ratio; hence, the results showed that inexpensive precipitation with ammonium sulfate can successfully be used as a purification method in the production of that plant coagulant in standardized form.
Idioma originalEnglish
Páginas (de-até)1746-1751
Número de páginas6
RevistaJournal of Food Science
Volume67
Número de emissão5
DOIs
Estado da publicaçãoPublished - 2002

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