TY - JOUR
T1 - KIF13A mediates trafficking of influenza Avirus ribonucleoproteins
AU - Ramos-Nascimento, Ana
AU - Kellen, Bárbara
AU - Ferreira, Filipe
AU - Alenquer, Marta
AU - Vale-Costa, Sílvia
AU - Raposo, Graça
AU - Delevoye, Cédric
AU - Amorim, Maria João
N1 - Funding Information:
This project and B.K. are supported by the project grant awarded by the Fundaçaõ para a Ciência e a Tecnologia (FCT) (Portugal: PTDC/IMI-MIC/1142/2012). M.J.A. is funded by the FCT investigator fellowship IF/00899/2013; M.A. and S.V.-C. by the FCT postdoc fellowships (SFRH/BPD/62982/2009 and SFRH/BPD/94204/2013, respectively); F.F. by the Instituto and Fundaçaõ Calouste Gulbenkian, Portugal. G.R. is supported by Fondation pour la Recherche Médicale (Equipe FRM DEQ20140329491 Team label), C.D. by the Fondation ARC pour la Recherche sur le Cancer (PJA20161204965), and both by Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM) and Institut Curie.
Publisher Copyright:
© 2017.
PY - 2017
Y1 - 2017
N2 - Influenza A is a rapidly evolving virus that is successful in provoking periodic epidemics and occasional pandemics in humans. Viral assembly is complex as the virus incorporates an eight-partite genome of RNA (in the form of viral ribonucleoproteins, vRNPs), and viral genome assembly - with its implications to public health - is not completely understood. It has previously been reported that vRNPs are transported to the cell surface on Rab11-containing vesicles by using microtubules but, so far, no molecular motor has been assigned to the process. Here, we have identified KIF13A, a member of the kinesin-3 family, as the first molecular motor to efficiently transport vRNP-Rab11 vesicles during infection with influenza A. Depletion of KIF13A resulted in reduced viral titers and less accumulation of vRNPs at the cell surface, without interfering with the levels of other viral proteins at sites of viral assembly. In addition, when overexpressed and following two separate approaches to displace vRNP-Rab11 vesicles, KIF13A increased levels of vRNP at the plasma membrane. Together, our results show that KIF13A plays an important role in the transport of influenza A vRNPs, a crucial step for viral assembly.
AB - Influenza A is a rapidly evolving virus that is successful in provoking periodic epidemics and occasional pandemics in humans. Viral assembly is complex as the virus incorporates an eight-partite genome of RNA (in the form of viral ribonucleoproteins, vRNPs), and viral genome assembly - with its implications to public health - is not completely understood. It has previously been reported that vRNPs are transported to the cell surface on Rab11-containing vesicles by using microtubules but, so far, no molecular motor has been assigned to the process. Here, we have identified KIF13A, a member of the kinesin-3 family, as the first molecular motor to efficiently transport vRNP-Rab11 vesicles during infection with influenza A. Depletion of KIF13A resulted in reduced viral titers and less accumulation of vRNPs at the cell surface, without interfering with the levels of other viral proteins at sites of viral assembly. In addition, when overexpressed and following two separate approaches to displace vRNP-Rab11 vesicles, KIF13A increased levels of vRNP at the plasma membrane. Together, our results show that KIF13A plays an important role in the transport of influenza A vRNPs, a crucial step for viral assembly.
KW - Influenza A virus assembly
KW - KIF13A
KW - Molecular motor
KW - Recycling endosome
UR - http://www.scopus.com/inward/record.url?scp=85036584504&partnerID=8YFLogxK
U2 - 10.1242/jcs.210807
DO - 10.1242/jcs.210807
M3 - Article
C2 - 29061883
AN - SCOPUS:85036584504
SN - 0021-9533
VL - 130
SP - 4038
EP - 4050
JO - Journal of Cell Science
JF - Journal of Cell Science
IS - 23
ER -